in TNFa-stimulated endothelial cells and PMA-stimulated HT-1080 cells (Hwang
et al. 2011; Kumar et al. 2007). However, piperine does not affect the IL-1b-induced
transcriptional activity of NF-jB in synoviocytes (Bang et al. 2009).
Piperine-mediated inhibition of the NF-jB pathway, therefore, appears to be cell
type-specific. We report here that piperine-treated DCs had decreased nuclear levels
of the NFjB subunit RelB, which is normally abundant in the nuclei of DCs matured
by stimulation with pathogen-associated molecular pattern molecules (Neumann
et al. 2000). Since RelB is critical for DC maturation and antigen-presenting function
(Zanetti et al. 2003) and NF-jB controls CCR7 transcript (Sánchez-Sánchez et al.
2006), and reduced RelB expression in the cores of piperine pickled DCs shows
partially for suppressive response for piperine in DC development, immigration, and
T cell excitation detected in the experiment. Though, piperine suppresses
mitogen-activated protein kinase signaling in numerous cells (Bang et al. 2009;
Hwang et al. 2011), including DCs (Bae et al. 2012). We suggest that
Fig. 22.10 Concentration-dependent effect of piperine on I K in PC-3 cells. A Representative
current traces of IK recorded in the absence and presence of piperine at different concentrations
(0.1, 25, 50 and 100 lM). B The mean I-V of I K in the absence and presence of piperine at
different concentration. Data are plotted as mean ± SEM (n > 7). With copyright permission
(George et al. 2019)
22 Piperine: Sources, Properties, Applications …
779
et al. 2011; Kumar et al. 2007). However, piperine does not affect the IL-1b-induced
transcriptional activity of NF-jB in synoviocytes (Bang et al. 2009).
Piperine-mediated inhibition of the NF-jB pathway, therefore, appears to be cell
type-specific. We report here that piperine-treated DCs had decreased nuclear levels
of the NFjB subunit RelB, which is normally abundant in the nuclei of DCs matured
by stimulation with pathogen-associated molecular pattern molecules (Neumann
et al. 2000). Since RelB is critical for DC maturation and antigen-presenting function
(Zanetti et al. 2003) and NF-jB controls CCR7 transcript (Sánchez-Sánchez et al.
2006), and reduced RelB expression in the cores of piperine pickled DCs shows
partially for suppressive response for piperine in DC development, immigration, and
T cell excitation detected in the experiment. Though, piperine suppresses
mitogen-activated protein kinase signaling in numerous cells (Bang et al. 2009;
Hwang et al. 2011), including DCs (Bae et al. 2012). We suggest that
Fig. 22.10 Concentration-dependent effect of piperine on I K in PC-3 cells. A Representative
current traces of IK recorded in the absence and presence of piperine at different concentrations
(0.1, 25, 50 and 100 lM). B The mean I-V of I K in the absence and presence of piperine at
different concentration. Data are plotted as mean ± SEM (n > 7). With copyright permission
(George et al. 2019)
22 Piperine: Sources, Properties, Applications …
779