yellow were measured at 517 nm. The percentage inhibition of DPPH free radical
scavenging activity was calculated using the following equation:
%Inhibition ¼ ½ðA DPPH À A Sample Þ=A DPPH à 100%
Where, A DPPH = Absorbance of DPPH, A Sample = Absorbance of sample (Bora
et al. 2019; Pal 2013; Nimse and Pal 2015).
Different hydrolysable tannins like geraniin, pentagalloyl glucose, chebulinic
acid, geraniin isoterchebin, mallotusinic acid, pedunculagin, pedunculagin, tellimagrandins I and II exert their anti-oxidant property by lipid peroxidation prevention interfering with xanthin-xanthin oxidase complex, adenine diphosphate and
ascorbic acid or with DPPH (Okuda 2005). Different types of condensed tannins
such as procyanidins B 1 and B 3 (Ariga et al. 1988) and kaki tannin also helps to
inhibit H 2 O 2 or Fe
2+ mediated lipid peroxidation (Jerez et al. 2007). Another study
using DPPH assay and oxygen radical absorbance capacity (ORAC) assays, showed
that Bengal coffee extract is having more potency towards anti-oxidant property
than Arabic coffee (Patay et al. 2016). Arsenic mediated liver injury and
obese-diabetic rat models are protected by administering tannin rich cocoa extract
for 4 weeks (Chandranayagam et al. 2013; Jalil et al. 2008). Different anti-oxidant
properties of tannins is listed below in Table 21.2.
Table 21.2 Anti-oxidant property of different tannins
Tannin
Characteristics
Assay
type
Geraniin, Pentagalloyl
glucose
Chebulinic acid,
geraniin
isoterchebin,
mallotusinic acid,
pedunculagin,
pentagalloylglucose,
tellimagrandins I and
II
Lipid peroxidation, interferes with xanthin-xanthin
oxidase complex, adenine diphosphate and ascorbic acid
and scavenging effects on DPPH free radical
In-vitro
Assay
Pedunculagin,
epigallocatechin
gallate
Lipid peroxidation in rat liver mitochondria stimulated
by adenine di-phosphate and ascorbic acid
Kaki-tannin
Significant inhibition of the auto-peroxidation of lipids
caused with H 2 O 2 or Fe
2+ /
Ascorbic acid
Tannin-rich cocoa
Arsenic mediated liver injury protection
In-vivo
Cocoa extracts
Anti-oxidant effects in the obese-diabetic rats
21 Tannins and Polyphenols Extracted from Natural Plants …
737
scavenging activity was calculated using the following equation:
%Inhibition ¼ ½ðA DPPH À A Sample Þ=A DPPH à 100%
Where, A DPPH = Absorbance of DPPH, A Sample = Absorbance of sample (Bora
et al. 2019; Pal 2013; Nimse and Pal 2015).
Different hydrolysable tannins like geraniin, pentagalloyl glucose, chebulinic
acid, geraniin isoterchebin, mallotusinic acid, pedunculagin, pedunculagin, tellimagrandins I and II exert their anti-oxidant property by lipid peroxidation prevention interfering with xanthin-xanthin oxidase complex, adenine diphosphate and
ascorbic acid or with DPPH (Okuda 2005). Different types of condensed tannins
such as procyanidins B 1 and B 3 (Ariga et al. 1988) and kaki tannin also helps to
inhibit H 2 O 2 or Fe
2+ mediated lipid peroxidation (Jerez et al. 2007). Another study
using DPPH assay and oxygen radical absorbance capacity (ORAC) assays, showed
that Bengal coffee extract is having more potency towards anti-oxidant property
than Arabic coffee (Patay et al. 2016). Arsenic mediated liver injury and
obese-diabetic rat models are protected by administering tannin rich cocoa extract
for 4 weeks (Chandranayagam et al. 2013; Jalil et al. 2008). Different anti-oxidant
properties of tannins is listed below in Table 21.2.
Table 21.2 Anti-oxidant property of different tannins
Tannin
Characteristics
Assay
type
Geraniin, Pentagalloyl
glucose
Chebulinic acid,
geraniin
isoterchebin,
mallotusinic acid,
pedunculagin,
pentagalloylglucose,
tellimagrandins I and
II
Lipid peroxidation, interferes with xanthin-xanthin
oxidase complex, adenine diphosphate and ascorbic acid
and scavenging effects on DPPH free radical
In-vitro
Assay
Pedunculagin,
epigallocatechin
gallate
Lipid peroxidation in rat liver mitochondria stimulated
by adenine di-phosphate and ascorbic acid
Kaki-tannin
Significant inhibition of the auto-peroxidation of lipids
caused with H 2 O 2 or Fe
2+ /
Ascorbic acid
Tannin-rich cocoa
Arsenic mediated liver injury protection
In-vivo
Cocoa extracts
Anti-oxidant effects in the obese-diabetic rats
21 Tannins and Polyphenols Extracted from Natural Plants …
737