(265) exhibited moderate cytotoxicity against BGC-823, HepG 2 , HL-60, and
MCF-7 cells, wherein IC 50 values were ranging in 16.9–24.2 µM. Adriamycin IC 50
values were observed in the range of 0.01–0.04 µM.
14.3.7 Phytochemistry and Pharmacological Activities
of A. Spatulata
14.3.7.1 Cytotoxicity Activity
Tan et al. isolated 23 (15, 17, 20, 29, 269–287) alkaloids from ethanolic extract of
A. spatulata leaves and stembark (Fig. 14.51) (Tan et al. 2010). Further, they
evaluated the cytotoxicity effects of these alkaloids against KB/S (vincristinesensitive) and KB/VJ300 (vincristine-resistant) cell lines. In KB/S cell lines,
15-hydroxyangustilobine A (269) and angustilobine B (281) exhibited a potential
activity (IC 50 = 5.26 and 6.99 µg/mL, respectively), while the remaining screened
compounds were found to be inactive (IC 50 > 25 µg/mL). 281 exhibited significant
cytotoxicity (IC 50 = 8.12 µg/mL). 24 and 269 exhibited moderate cytotoxicity
(IC 50 = 13.35 and 14.39 µg/mL, respectively). However, except alstolucine C
(278), nor-6,7-secoangustilobine A (283), and undulifoline (284), all the tested
compounds were found to reverse MDR in vincristine-resistant KB (VJ300) cells
(IC 50 = 0.59–19.22 µg/mL).
Alstiyunnanenine A (263)
Alstiyunnanenine B (264);
R = veratoryl
Alstiyunnanenine C (265)
Alstiyunnanenine D (266)
Alstiyunnanenine E (267)
Alstoniascholaine I (268)
N
N
OH
O
H
OMe
H
N
N
H
O
COOMe
RO
N
N
H
O
COOMe
H
N
H
N
O
COOH
OH
H
H
HO
N
H
N
COO
OH
H
H
HO
N
H
N
O
COOH
H H
O
H
H
H
OH
Fig. 14.50 Chemical structures of various alkaloids from A. yunnanensis (263–268)
14 Pharmaceutical Application of Bio-actives …
511