Chapter 8
Capillary Electrophoresis: A New
Evolutionary Platform of Plant
Secondary Metabolites
Dilipkumar Pal and Souvik Mukherjee
Abstract Capillary electrophoresis (c.e) is a modern S pt technique other than any
other chromatographic technique for the detection of plant secondary metabolites
(Psm). There are two types of c.e such as capillary zone electrophoresis (c.z.e) and
micellar electro kinetic (M.e.k.c.c). All kinds of secondary metabolites (S m ) (flavonoids, cardiac glycosides, aglycones, steroids, diterpene, saponin, etc.) are not
possible to isolate with the help of HPLC and Gas chromatography. But such type
of metabolites may possibly be isolated with the help of c.e smoothly and easily.
However, in c.e charged molecules are transferred to the opposite charged molecules in the presence of the electrical field. Very low solvent, low price silica
columns, and small amount of samples are needed to run the c.e. There are various
characteristics such as voltage (V tg ), temperature, electrolyte concentration, B F pH,
micelle concentration, and organic modifiers may influence the S pt of different S m .
In this book chapter we will describe the different parameters of c.e like methodology, detector sample analysis, and combination of other hypnated technique for
the detection of metabolites.
Keywords Capillary zone electrophoresis Á Micellar electro kinetic capillary
chromatography Á Phenolics Á Alkaloids Á Terpenoids
Abbreviation
A NT
Acetonitrile
Alkd
Alkaloid
A ACT
Ammonium acetate
BRT
Borate
B F
Buffer
BFS
Buffer solution
C.e
Capillary electrophoresis
C.w
Capillary wall
D. Pal (&) Á S. Mukherjee
Department of Pharmaceutical Sciences, Guru Ghasidas Viswavidyalaya
(A Central University), Bilaspur, Chhattisgarh 495 009, India
e-mail: drdilip2003@yahoo.co.in
© The Author(s), under exclusive license to Springer Nature Switzerland AG 2021
D. Pal and A. K. Nayak (eds.), Bioactive Natural Products
for Pharmaceutical Applications, Advanced Structured Materials 140,
https://doi.org/10.1007/978-3-030-54027-2_8
287
Capillary Electrophoresis: A New
Evolutionary Platform of Plant
Secondary Metabolites
Dilipkumar Pal and Souvik Mukherjee
Abstract Capillary electrophoresis (c.e) is a modern S pt technique other than any
other chromatographic technique for the detection of plant secondary metabolites
(Psm). There are two types of c.e such as capillary zone electrophoresis (c.z.e) and
micellar electro kinetic (M.e.k.c.c). All kinds of secondary metabolites (S m ) (flavonoids, cardiac glycosides, aglycones, steroids, diterpene, saponin, etc.) are not
possible to isolate with the help of HPLC and Gas chromatography. But such type
of metabolites may possibly be isolated with the help of c.e smoothly and easily.
However, in c.e charged molecules are transferred to the opposite charged molecules in the presence of the electrical field. Very low solvent, low price silica
columns, and small amount of samples are needed to run the c.e. There are various
characteristics such as voltage (V tg ), temperature, electrolyte concentration, B F pH,
micelle concentration, and organic modifiers may influence the S pt of different S m .
In this book chapter we will describe the different parameters of c.e like methodology, detector sample analysis, and combination of other hypnated technique for
the detection of metabolites.
Keywords Capillary zone electrophoresis Á Micellar electro kinetic capillary
chromatography Á Phenolics Á Alkaloids Á Terpenoids
Abbreviation
A NT
Acetonitrile
Alkd
Alkaloid
A ACT
Ammonium acetate
BRT
Borate
B F
Buffer
BFS
Buffer solution
C.e
Capillary electrophoresis
C.w
Capillary wall
D. Pal (&) Á S. Mukherjee
Department of Pharmaceutical Sciences, Guru Ghasidas Viswavidyalaya
(A Central University), Bilaspur, Chhattisgarh 495 009, India
e-mail: drdilip2003@yahoo.co.in
© The Author(s), under exclusive license to Springer Nature Switzerland AG 2021
D. Pal and A. K. Nayak (eds.), Bioactive Natural Products
for Pharmaceutical Applications, Advanced Structured Materials 140,
https://doi.org/10.1007/978-3-030-54027-2_8
287