582
A. Singhal et al.
Fig. 3 Schematic Illustration of the TWJs-based sensing platform used for the DNA, Thrombin,
and ATP analysis, respectively; reproduced with permission from Lubin and Plaxco (2010)
DNA-based nanobio-constructs that are effective for dye adsorption, retrieval, and
reuse.
2 Materials and Methods
5
-amino or 5
-biotin labeled oligonucleotides were purchased from IDT (Bangalore,
India). Unmodified oligonucleotides were purchased from Sigma (Bangalore, India).
Stock solutions were prepared in Tris-EDTA (pH 7.5) buffer. Stock solutions
(~100 μM) and 260/280 ratio of the stock were calculated using Thermo Scientific
Nanodrop 2000c and stored at −20 °C for further use. Mili-Q water was used for all
preparations. Nucleic acid sequences used in the work are as follows:
Name
Oligonucleotide sequence
Modification
A1N
GGTGAGGTGGCGAGAGCGACGATCCATGCC
NH 2
S2N
GGCATGGATCGTCGCAGAGTTGACCTGACC
NH 2
S3N
GGTCAGGTCAACTCTTCTCGCCACCTCACC
NH 2
B1S2-10
GGCATGGATC
Biotin
B1S3-10
GGTCAGGTCA
Biotin
(continued)
A. Singhal et al.
Fig. 3 Schematic Illustration of the TWJs-based sensing platform used for the DNA, Thrombin,
and ATP analysis, respectively; reproduced with permission from Lubin and Plaxco (2010)
DNA-based nanobio-constructs that are effective for dye adsorption, retrieval, and
reuse.
2 Materials and Methods
5
-amino or 5
-biotin labeled oligonucleotides were purchased from IDT (Bangalore,
India). Unmodified oligonucleotides were purchased from Sigma (Bangalore, India).
Stock solutions were prepared in Tris-EDTA (pH 7.5) buffer. Stock solutions
(~100 μM) and 260/280 ratio of the stock were calculated using Thermo Scientific
Nanodrop 2000c and stored at −20 °C for further use. Mili-Q water was used for all
preparations. Nucleic acid sequences used in the work are as follows:
Name
Oligonucleotide sequence
Modification
A1N
GGTGAGGTGGCGAGAGCGACGATCCATGCC
NH 2
S2N
GGCATGGATCGTCGCAGAGTTGACCTGACC
NH 2
S3N
GGTCAGGTCAACTCTTCTCGCCACCTCACC
NH 2
B1S2-10
GGCATGGATC
Biotin
B1S3-10
GGTCAGGTCA
Biotin
(continued)