H 2 /mol glucose which was produced at 13% NaCl, initial pH 6.8, 35
C, and food to
microorganism ratio of 1.5 after 1 year of acclimatization.
Although dark fermentation with extremely halotolerant bacteria could offer an
affordable process for hydrogen production, the pretreatment cost of the lignocellulosic biomass remains a challenge until now. This research focused on the dark
fermentation process with the isolated extremely halotolerant hydrogen-producing
bacteria from the salt pan and salt-damaged soil and did not cover the part of
lignocellulosic biomass pretreatment process. Figure 11.5 shows all the processes
involved in biohydrogen production from lignocellulosic biomass. A technology
roadmap for biohydrogen production from lignocellulosic biomass can be found in
Fig. 11.6.
Fig. 11.6 Biohydrogen production from lignocellulosic biomass technology roadmap. * 1US
$¼107.5 Japanese Yen (May 2020)
Lignocellulosic
biomass
Pretreatment
(physical/chemical/
biological)
Neutralization/
detoxification
Enzymatic hydrolysis
Biohydrogen
production (dark
fermentation of
sugars)
Gas purification &
separation of VFAs
Fig. 11.5 Processes in biohydrogen production from lignocellulosic biomass. The box in gray and
bold text shows the part covered in this research
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D. A. H. Taroepratjeka et al.