Table 1
Experimental design used to optimize stage 1 (37 ˚C) of the total acid hydrolysis methodology for
quantifying carbohydrates in seaweed
Run
Factor 1
Factor 2
A: H 2 SO 4 acid concentration (M)
B: time at 37
C (min)
1
1 2
1 5
2
3
1 5
3
3
1 5
4
7
1 5
5
7
3 5
6
3
6 0
7
5
4 5
8
1 2
3 5
9
7
3 5
10
5
25
11
12
60
12
10
25
13
3
35
14
3
60
15
12
15
16
7
60
17
12
60
18
7
35
19
7
35
20
10
45
21
5
60
22
10
60
23
10
15
24
12
60
25
12
60
26
12
15
27
12
15
28
8
15
29
8
15
30
8
60
(continued)
186
Emily T. Kostas et al.