Table 1
A summary of studies aimed at increasing TAG formation in various microalgae species by
overexpression of GPAT or LPAAT genes
Microalgal
species
Type of manipulation
Results
Ref.
Cyclotella cryptica Overexpression of ACCase gene
No effect
[29]
Navicula
saprophila
Overexpression of ACCase gene
No effect
[29]
Scenedesmus
quadricauda
Overexpression of ACCase gene
1.6-fold improvement in fatty
acid content
[31]
Nannochloropsis
oceanica
Overexpression of MAT
36% improvement in lipid
content
[77]
Schizochytrium
Overexpression of MAT
39.6% improvement in lipid
content
[78]
Phaeodactylum
tricornutum
Overexpression of KAS
No effect
[33]
Phaeodactylum
tricornutum
Overexpression of LPAAT
Improvement in lipid
accumulation
[79]
Chlamydomonas
reinhardtii
Overexpression of LPAAT
20% improvement in lipid
content
[80]
Phaeodactylum
tricornutum
Expression of GPAT under the Pt211
promoter
2.6-fold improvement in lipid
content
[81]
Phaeodactylum
tricornutum
Expression of GPAT
2 fold improvement in lipid
content
[82]
Chlamydomonas
reinhardtii
Overexpression of GPAT
Above 50% improvement in
TAG content
[83]
Phaeodactylum
tricornutum
Overexpression of DGAT1
35% improvement in neutral
lipid content
[41]
Nannochloropsis
oceanica
Overexpression of DGAT1
47% improvement in TAG
content
[84]
Chlamydomonas
reinhardtii
Overexpression of DGAT2
9 fold improvement in TAG
content
[43]
Chlamydomonas
reinhardtii
Overexpression of DGAT2
29-fold improvement in TAG
content
[85]
Scenedesmus
obliquus
Overexpression of the DGAT2 from
Chlamydomonas reinhardtii
84.9% improvement lipid
content
[42]
Nannochloropsis
oceanica
Overexpression of DGAT2
78% improvement in TAG
content
[86]
Nannochloropsis
oceanica
Overexpression of DGAT2
69% improvement in neutral
lipid content
[87]
Chlorella
minutissima
Overexpression of G3PDH, GPAT, LPAAT,
PAP, and DGAT
2 fold improvement in neutral
lipid content
[88]
(continued)
158
Mohammad Pooya Naghshbandi et al.