492
was evaluated with streptomycin serving as a control. The result obtained shows
that the aqueous extract obtained from the various beans exhibited antimicrobial
activity against all the tested bacteria. The antibacterial was shown to be bacteriostatic. The antibacterial activity on E. coli varies from 9 mm to 15 mm while B. subtilis varies from 10 to 12 mm.
Küçükboyacı et al. (2012), utilized gas chromatography-mass spectrometry to
determine various alkaloids available in the Genista sandrasica Hartvig & Strid
which belong to Fabaceae family grown in Turkey. The percentages of ten quinolizidine alkaloid detected in the plant include anagyrine (40.49%), Nacetylcytisine
(6.48%), baptifoline (10.76%), and 13-methoxylupanine (13.12%) respectively.
The antimicrobial effectiveness of the alkaloid extract of Genista sandrasica was
carried out against the following microorganisms which include Pseudomonas
aeruginosa, Candida krusei, Staphylococcus aureus, Escherichia coli, Candida
albicans, and Bacillus subtilis. The minimum inhibitory concentration exhibited by
the crude extract from Genista sandrasica against S. aureus and B. subtilis was
62.5 μg/mL and 31.25 μg/mL respectively.
Hossain et al. (2012), evaluated the biological activity of Cassia senna leaves
including its antimicrobial effectiveness which belongs to Fabaceae family. The
crude extract present in Cassia senna leaves was extracted using chloroform, methanolic, n-hexane, ethyl acetate respectively. The crude extract was tested against
8-gram (−) bacteria, three fungi and 5-gram (+) bacteria using disc diffusion
method. The result shows that n-hexane and chloroform fractions exhibited some
level of antimicrobial effect when compared to the methanol extract without any
antimicrobial effect.
Pahune et al. (2013) validated the antimicrobial effect of Clitoria ternatea Linn
which is a species under a genus Clitoria, family Fabaceae. The main aim of their
study was to evaluate the utilization of Clitoria ternatea Linn as a natural indicator
as a permanent replacement to synthetic indicator. The natural indicator will be
utilized in numerous acid-base titration. The result reveals that the equivalence
points observed from the flower are comparable to the one by the standard indicators. Moreover, the same result was obtained from the weak base and weak acid
titration most especially from the flower extract. Furthermore, the methanolic flower
extract exhibits a significant antimicrobial effect against Staphylococcus aureus.
Their research showed that natural indicator is more effective, simple, accurate and
economical and eco-friendly when compared to the synthetic indicator most especially for acid-base titration.
Dzoyem et al. (2014) evaluated the effect of acetone leaf extracts of nine medicinal plant that belongs to the family of Fabaceae that has been under-investigated.
The antimicrobial activity was performed using a serial microdilution against three
Gram-negative and against three Gram-positive bacteria. Moreover, it was observed
that 6 out of the 9 acetone extracts exhibited a significant antimicrobial activity
with minimum inhibitory concentration values that varies from MIC 20–80 μg/mL
against all the tested bacterial species. Among all the plant extract tested, Crotalaria
capensis showed the highest activity against Salmonella typhimurium followed by
Indigofera cylindrical, with minimum inhibitory concentration value that ranges
S. Das et al.
was evaluated with streptomycin serving as a control. The result obtained shows
that the aqueous extract obtained from the various beans exhibited antimicrobial
activity against all the tested bacteria. The antibacterial was shown to be bacteriostatic. The antibacterial activity on E. coli varies from 9 mm to 15 mm while B. subtilis varies from 10 to 12 mm.
Küçükboyacı et al. (2012), utilized gas chromatography-mass spectrometry to
determine various alkaloids available in the Genista sandrasica Hartvig & Strid
which belong to Fabaceae family grown in Turkey. The percentages of ten quinolizidine alkaloid detected in the plant include anagyrine (40.49%), Nacetylcytisine
(6.48%), baptifoline (10.76%), and 13-methoxylupanine (13.12%) respectively.
The antimicrobial effectiveness of the alkaloid extract of Genista sandrasica was
carried out against the following microorganisms which include Pseudomonas
aeruginosa, Candida krusei, Staphylococcus aureus, Escherichia coli, Candida
albicans, and Bacillus subtilis. The minimum inhibitory concentration exhibited by
the crude extract from Genista sandrasica against S. aureus and B. subtilis was
62.5 μg/mL and 31.25 μg/mL respectively.
Hossain et al. (2012), evaluated the biological activity of Cassia senna leaves
including its antimicrobial effectiveness which belongs to Fabaceae family. The
crude extract present in Cassia senna leaves was extracted using chloroform, methanolic, n-hexane, ethyl acetate respectively. The crude extract was tested against
8-gram (−) bacteria, three fungi and 5-gram (+) bacteria using disc diffusion
method. The result shows that n-hexane and chloroform fractions exhibited some
level of antimicrobial effect when compared to the methanol extract without any
antimicrobial effect.
Pahune et al. (2013) validated the antimicrobial effect of Clitoria ternatea Linn
which is a species under a genus Clitoria, family Fabaceae. The main aim of their
study was to evaluate the utilization of Clitoria ternatea Linn as a natural indicator
as a permanent replacement to synthetic indicator. The natural indicator will be
utilized in numerous acid-base titration. The result reveals that the equivalence
points observed from the flower are comparable to the one by the standard indicators. Moreover, the same result was obtained from the weak base and weak acid
titration most especially from the flower extract. Furthermore, the methanolic flower
extract exhibits a significant antimicrobial effect against Staphylococcus aureus.
Their research showed that natural indicator is more effective, simple, accurate and
economical and eco-friendly when compared to the synthetic indicator most especially for acid-base titration.
Dzoyem et al. (2014) evaluated the effect of acetone leaf extracts of nine medicinal plant that belongs to the family of Fabaceae that has been under-investigated.
The antimicrobial activity was performed using a serial microdilution against three
Gram-negative and against three Gram-positive bacteria. Moreover, it was observed
that 6 out of the 9 acetone extracts exhibited a significant antimicrobial activity
with minimum inhibitory concentration values that varies from MIC 20–80 μg/mL
against all the tested bacterial species. Among all the plant extract tested, Crotalaria
capensis showed the highest activity against Salmonella typhimurium followed by
Indigofera cylindrical, with minimum inhibitory concentration value that ranges
S. Das et al.