the second sample (sample B; fresh weight 4 mg): the relative plate count of the tenth
wash fraction and the ultrasonicated fraction were 0.5% and 24%, respectively, of
the total recovered CFU from the combined wash and ultrasonicated fractions.
Following the same procedure, sample C (fresh weight, 9 mg) was washed ten
times and then bacteria were isolated from the ultrasonicate and the first wash
Sclerotia
Washing fluid
Sterilized water
Sterilized
water
Sclerotia suspension
Washing treatment
10 times
After washing treatment
Inoculate external
bacteria on a culture
plate
Inoculate internal
bacteria on a culture
plate
Fig. 4.2 The washing procedure for isolating bacteria from sclerotia grains
No.of washes
Percentage of plate counts
Ultras
onication
2 3
6
8
100
0
20
40
60
80
0 1
4 5
7
9 10
Fig. 4.3 Plate counts of repeated wash solutions and ultrasonicates of sclerotia grains from Myoko
soil. Sclerotia samples (fresh weight): filled circle, (weight not determined); open circle, (4 mg);
inverted filled triangle, (9 mg) (Ohta et al. 2003)
4 Sclerotia Grains as Bacterial Carriers in Soil
67