Table 3
Existing differences in the literature regarding feeding behavioural experiments
Leaf species
Contaminant
Water used
for the
experiment
Light/dark
cycle
Leaf disc size
(diameter)
Length of the
feeding study
Drying temperature and
time (before and after
exposure)
Starvation Equation used
References
Aesculus
hippocastanum
Imidacloprid
Artificial
pond water
12:12
1.6 cm
3 at the time
and exchanged
every 24 h
7 days
(4 days
exposure
+3 days
recovery
phase)
Leaves:
Before: 60
C for 48 h
After:
Organisms:
After: 65
C for 48 h
FR
¼
F tÀ1
½
Š À
F t
½ Š
ld
GÂt
FR
¼ feeding rate
F[t
À 1]
¼ initial
leaf dry weight (mg)
F[t]
¼
final leaf dry weight (mg)
G
¼ dry weight of Gammarus (mg)
ld
¼ leaching decomposition factor
t
¼ time (days)
Agatz et al.
(2014)
1. Aesculus
hippocastanum
2. Alnus
glutinosa
Artificial
pond water
12:12
1. 1.6 cm
2. 2.0 cm
Experiment
1:
96 h
Experiment
2:
9 days
Experiment
3:
15 days
Leaves:
1.
2.
Before: 60
C to constant
weight
After:
Organisms:
After: 90
C for 24 h
Wet weight transform in
dry weight using a linear
regression
FR
¼
F tÀ1
½
Š À
F t
½ Š
ld
GÂt
FR
¼ feeding rate
F[t
À 1]
¼ initial
leaf dry weight (mg)
F[t]
¼
final leaf dry weight (mg)
G
¼ dry weight of Gammarus (mg)
ld
¼ leaching decomposition factor
t
¼ time (days)
Agatz and
Brown
(2014)
Alnus
glutinosa
Microplastic
debris
Mixed water
(municipal
water and
softened
water)
2.0 cm
2 discs at the
time every
7 days
28 days
Leaves:
Before: 60
C for 24 h
After: 60
C for 24 h
FR
¼
Li
 CF
ð ÞÀLf
wÂt
FR
¼ feeding rate
L
i ¼ initial leaf dry weight (mg)
L
f ¼
final leaf dry weight (mg)
w
¼ animal
0
s average wet weight (mg)
t
¼ feeding time (days)
CF
¼ leaf change correction factor
CF
¼
P C
f
Ci
h
i
n
C
f ¼
final dry weight of control leaf
discs (mg)
C
i ¼ initial dry weight of control leaf
discs (mg)
n
¼ number of replicates
Blarer and
BurkhardtHolm
(2016)
(continued)
Feeding Behavioural Studies with Freshwater Gammarus spp.: The. . .
25