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S. Adeel et al.
between mechanical agitation and cellulase activity is vital for pill removing without
any strength loss to get valuable fuzz fiber [72]. Enzymatic softening or bio-polishing
of fabric lasts over several washes [73].
3.1.7 Bio-mercerizing
The next step to improve dye affinity, luster and strength of the fabric is mercerizing
which involves conventional process i.e., treatment with NaOH [74]. It is not a fiber
purification process, but it does induce desirable changes in cotton yarn and fabric
properties [75]. Mercerization is a well-established finishing process for cotton which
affects its fine structure, morphology, mechanical properties and reactivity [76]. This
step is usually performed after bleaching on gray cloth [77]. For bio-mercerizing
few enzymes like proteases, lipases, pectinases, and cellulases have been reported
for mercerizing process yet detailed studies are needed [78].
3.1.8 Bio-dyeing
Enzymes are continuously used since the last decade for bio-preparation of silk,
leather, wool and cotton fabric [79]. Diastasis, lipases proteases, and amylase, all
four enzymes in the conjugation of tannic acid enhance the dye-ability [80]. Diastasis
with natural colorant like Punicagranatum (pomegranate), lipase with Rheum emodi
(red wined pie plant), amylase and proteases with Terminalia arjuna (Arjun tree)
produced best colorimetric picture regarding dyeing [81]. Markedly, two well-known
textiles Sir Naturals and Jet Hosiery have established a sonicator for dyeing cotton
and silk naturally on the industrial level [2].
3.1.9 Bio-color Stripping
Destructive stripping, back stripping or color stripping is a dye removal process.
Enzymes belong to oxidoreductases like lignin peroxidase, laccases, manganese peroxidase perform color stripping using mechanisms of decolorization, bioremediation,
mineralization or degradation [82–85] Color stripping potential of different microbial strains with non-specific nature of enzymes has also been investigated [86].
Indigenously isolated species of white-rot fungi i.e., Ganoderma lucidum exhibited
an excellent color stripping potential reactive black dyed cotton fabric [87]. Although
bio-stripping is eco-friendly with better results than chemical color stripping yet biostripping required larger time [88]. Limitation of time can be overcome by the use
of different biotechnological tools.
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