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Topics in Current Chemistry (2018) 376:43
When cross-linked with dimethyl suberimidate, the isolated metabolon exhibited a 50%
increase in catalytic current density over the free enzymes in solution.
As an extension of this work, Wu et al. were able to employ this same cross-linking
strategy to isolate the  eight enzyme components from the Kreb’s cycle and identify
interfacial amino acid residues between mitochondrial malate dehydrogenase, citrate
synthase, and aconitase [43]. This led to the discovery of two electrostatic pathways
O
OH
OH
OH
HO
OH
O
OH
OH
OH
HO
2- O 3 PO
O
2- O 3 PO
OH
OH
HO
OPO 3
22- O 3 PO
O
OH
2- O 3 PO
OH
O
2- O 3 PO
OPO 3
2OH
O
O
O
OPO 3
2O
O
O
O
2- O 3 PO
OH
OH
HO
OPO 3
22- O 3 PO
OH
OPO 3
2O
O
OH
OH
OH
HO
O
O
O
OH
OH
HO
OH
O
OH
O
O
HO
OH
O
O
O
OH
HO
OH
HO
O
OH
HO
OH
OH
O
O
HO
O
O
HO
OH
O
O
O
HO
OH
OH
O
OH
OH
OH
HO
O
O
O
O
O
HO
OH
O
OH
OH
OH
HO
OH
HO
O
OH
OH
O
HO
OH
TEMPO-LPEI 31
bGOx 20 ,
AuNPs 32
PDH 19
PDH 19
PDH 19
PDH 19
bGOx 20
HK
ATP
ADP
GPI
ATP
ADP
PFK1
ALD
TPI
ADP
ATP
PGK1
NAD
+
NADH
G3PDH
PGM,
enolase
PK
ADP
ATP
TEMPO-NH 2
27
TEMPO-LPEI 31
ADH/ALDH 14
TEMPO-NH 2
27
ADH 14
OH
CO 2
OxO 27
OxDC 29
FDH 28
MWCNT-COOH 30
OxO 27
OxDC (via formate)
29
(to Kreb's cycle)
GLYCOLYTIC PATHWAY
ELECTROMETABOLIC PATHWAY
1x 2 e - oxidation
total 24 e - oxidation
Fig. 7 Comparison between the native glycolytic pathway and a combination of electrometabolic pathways used to convert chemical energy (from glucose) into electrochemical energy
Reprinted from the journal
161