Topics in Current Chemistry (2018) 376:43
1 3
5.1x increase in overall cascade rate (as measured by NADH production) over the
freely diffusing enzymes. In addition to artificial scaffolds, researchers have taken
advantage of naturally occurring enzyme interactions to spatially orient sequential
proteins relative to each other.
Moehlenbrock et al. demonstrated the adaptation of such naturally occurring interactions in the design of an electroenzymatic cascade based on a portion of the Kreb’s
cycle [42]. The sequential enzymes of the Kreb’s cycle were covalently cross-linked
within extracted mitochondria to ensure that the proteins were linked in a conformation that mimics their native metabolon complex (Fig. 6). The cross-linked metabolon complex was isolated from lysed mitochondria and immobilized onto an electrode.
Fig. 6 An artificially cross-linked metabolon consisting of succinate dehydrogenase (SDH), fumarase
(FUM), and malate dehydrogenase (MDH)
Reprinted from the journal
160
1 3
5.1x increase in overall cascade rate (as measured by NADH production) over the
freely diffusing enzymes. In addition to artificial scaffolds, researchers have taken
advantage of naturally occurring enzyme interactions to spatially orient sequential
proteins relative to each other.
Moehlenbrock et al. demonstrated the adaptation of such naturally occurring interactions in the design of an electroenzymatic cascade based on a portion of the Kreb’s
cycle [42]. The sequential enzymes of the Kreb’s cycle were covalently cross-linked
within extracted mitochondria to ensure that the proteins were linked in a conformation that mimics their native metabolon complex (Fig. 6). The cross-linked metabolon complex was isolated from lysed mitochondria and immobilized onto an electrode.
Fig. 6 An artificially cross-linked metabolon consisting of succinate dehydrogenase (SDH), fumarase
(FUM), and malate dehydrogenase (MDH)
Reprinted from the journal
160