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2 High-Efficient Anaerobic Fermentation Technology of Organic …
Fig. 2.3 Impacts of different hydrothermal hydrolysis pre-treatments on floating oil and crude fat
2.2.1.3 Impacts of Hydrothermal Pre-treatment on Saccharides
Saccharide, as an important carbon source for the growth of microorganisms, can
be used directly or transformed into other raw materials or energy substances and is
mainly produced by hydrolysis of carbohydrates. When carbohydrates collide with
hydronium ion (H 3 O
+ ) or hydroxy radical (OH
− ), glycosidic bonds fracture to form
the corresponding monosome, namely, reducing sugar. The research demonstrated
that the content of reducing sugar in the experimental group treated at 80 and 120 °C
decreased, because low temperature resulted in the low hydration and ionisation
degree and material system was diluted after adding water. However, the content of
reducing sugar in the experimental group treated at 150 and 200 °C rose. The content
of reducing sugar added with 40% of water and treated for 60 min at 150 °C was
the highest, 0.257 g/L, which increased by 16.29% in comparison with the control
group (Fig. 2.4). Obviously, in hydrothermal system of hydrothermal pre-treatment,
the higher the temperature was, the greater the average kinetic energy of molecular
motion, the higher the hydration and ionisation degree and the stronger the effects on
glycosidic bonds. When the treatment temperature was above 150 °C, the increase of
liquefaction degree of carbohydrates raised the content of reducing sugar. The total
sugar showed a same change trend with reducing sugar and increased by 38.92%
in comparison with the control group. The total sugar mainly included reducing
sugar and non-reducing sugar. In non-reducing sugar, starch, sucrose and cellulose
generated saccharification reaction under the effects of thermal hydrolysis to form
soluble monosaccharides and oligosaccharides. On the one hand, saccharides were
important organic substrates in biotreatment. On the other hand, saccharides were
important energy substances in life activities of microorganisms, while metabolic
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