2.3 Field Methods
73
to record in the Notes section of the Water-Sampling Record Form the volume
of water that was filtered for chlorophyll a measurement.
2.3.4 Postcollection Sample Processing,
Documentation, and Cleanup
Filled sample bottles should be placed in zipper-lock bags prior to transport
from the field site. Syringes should be packed in a generic lightweight plastic
box with snap-on lid. Each box needs to be long enough to hold syringes that
are two-thirds to three-quarters filled with sample water and wide enough to
hold multiple syringes. The bagged sample bottles and boxed syringes should
be packed with double-bagged ice or frozen refrigerant for transport.
Insulated containers, with double-bagged chemical refrigerant (“blue ice”),
or preferably with double-bagged ice blocks, are needed for transport of collected samples between the field and other staging location and eventually to
the laboratory. Ice works better for shipping unless large numbers of chemical
refrigerant packs are used. Small insulated containers that will fit into backpacks can be used to carry and protect the samples in the field. Coolers can be
used for assembly and transport of samples in vehicles.
Chemical refrigerant containers should be packaged in two zipper-lock plastic bags to minimize the possibility of sample bottle contamination through
leakage. The field crews will need to make sure that the chemical refrigerant is
placed in a freezer at least 2 days before sampling.
2.3.4.1 Sample Documentation
Sample documentation should be completed in the field. It will include completing and affixing all sample labels, completing all field and chain-of-custody
forms, and recording field notes and site condition information. A list of suggested minimum database requirements for studies of the effects of atmospheric deposition are provided in Table 2.9. Documentation should also include
review of sampling procedures, labeling, and photographic/written documentation (Table 2.10).
2.3.4.2 Postsampling Equipment Cleanup
Cleanup is important to minimize the possibility of transporting pathogens,
noxious species, or invasive species from one sampling location to another.
Risks vary from region to region and location to location. Field personnel
should consult with regional and local land management offices for specific
problem identification and appropriate precautions and cleaning protocols.
A variety of forest pathogens and aquatic and terrestrial invasive species may
be of concern, depending on location. Personnel and their boots, vehicles,
boats, and equipment can serve as transport vehicles for problematic species.
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