8. Loss on drying : 10% ______________________________________________ 43
Vii. Antimicrobial activity of chitosan ________________________________________ 44
1. Disk diffusion test ___________________________________________________ 45
2. Mueller hinton agar : preparation / composition __________________________ 46
3. Microorganisms _____________________________________________________ 46
4. Preparation of inoculum ______________________________________________ 47
5. Chitosan sample solutions preparation __________________________________ 47
6. Procedure of antimicrobial testing _____________________________________ 48
A. Inoculation of the mh plate __________________________________________ 48
B. Placement of the sterile paper disks __________________________________ 48
Viii. Chitosan-based products production _____________________________________ 49
1- Wound healing – anti-inflammatory cream ______________________________ 49
Ix. In vivo wound healing experimentation ___________________________________ 51
A) Animal selection for in vivo experiment _________________________________ 51
B) Material ___________________________________________________________ 52
C) Wounding procedure ________________________________________________ 52
D) Skin irritation test ___________________________________________________ 52
E) Burn-wound procedure_______________________________________________ 54
X. Aqueous solution for plant propagation and protection ______________________ 55
Xi. Chitosan films for food preservation: _____________________________________ 55
Xii. Treatment of paper with chitosan hydrochloride: ___________________________ 55
Chapter 03 :Results and discussion
Iv. Optimization and evaluation of chitin and chitosan extraction methods: ________ 57
V. Chitin and chitosan characterization results _______________________________ 59
1- Chitosan yield ______________________________________________________ 59
2- Moisture content ____________________________________________________ 60
3- Water and fat binding capacity ________________________________________ 61
4- Protein identification test _____________________________________________ 62
5- Solubility___________________________________________________________ 63
6- Fourier-transform infrared spectroscopy (ft-ir) analysis ___________________ 64
7- Degree of deacetylation of chitosan _____________________________________ 65
8- Viscosity ___________________________________________________________ 66
9- Molecular weight ____________________________________________________ 66
Vi. Characterization of chitosan hydrochloride: european pharmacopoeia 6th edition
analysis
67