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b) Material
• Albino rabbits (2 males- (1.9-2kg))
• Chitosan-based anti-inflammatorycream
• Chitosan-based wound healing cream
• Anaesthetic agent
• Sterilized surgical instruments (scissors, forceps)
• Sterile gauze
• Disposable gloves and masks
• Antiseptic solution
• Adhesive tape
• Shaving machine or razor
c) wounding Procedure
• Put on gloves, a surgical mask, Clean the surgical instruments and the work surface with
an antiseptic solution, Position the rabbit in a ventral position (FIGURE 43-A), with
the dorsal exposed, then shave the area around the wound site, and disinfect the area
with an antiseptic solution.
• Apply Local anaesthesia in the area before the procedure about 30 min to minimize pain
(lidocaine 2.5 %, prilocaine 2.5%), Using a sterile scalpel blade, make a controlled
incision through the superficial layer of the skin (FIGURE 43-B), The wound will be
allowed to bleed to ensure adequate vascularity.
• Use sterile gauze or surgical suction to control any bleeding from the wound site. Apply
pressure with a sterile gauze or bandage to stop any bleeding, if necessary.
• For the cream application, a sequential application procedure was employed, wherein
an initial three-day treatment period involved the administration of a chitosan based
anti-inflammatory cream, followed by a subsequent application of a chitosan based
wound healing cream. Apply the cream to the wound site (FIGURE 43-C). Use a sterile
applicator to ensure cleanliness.
• After the procedure, the rabbits were housed in a clean, comfortable environment with
appropriate temperature, humidity, and ventilation, food and water as is also provided.
d) Skin irritation Test
After applying the cream, the wound site is covered with a sterile gauze patch. The first patch
is removed after 5-10 min. If no serious skin reaction is observed, a second patch will be placed.
b) Material
• Albino rabbits (2 males- (1.9-2kg))
• Chitosan-based anti-inflammatorycream
• Chitosan-based wound healing cream
• Anaesthetic agent
• Sterilized surgical instruments (scissors, forceps)
• Sterile gauze
• Disposable gloves and masks
• Antiseptic solution
• Adhesive tape
• Shaving machine or razor
c) wounding Procedure
• Put on gloves, a surgical mask, Clean the surgical instruments and the work surface with
an antiseptic solution, Position the rabbit in a ventral position (FIGURE 43-A), with
the dorsal exposed, then shave the area around the wound site, and disinfect the area
with an antiseptic solution.
• Apply Local anaesthesia in the area before the procedure about 30 min to minimize pain
(lidocaine 2.5 %, prilocaine 2.5%), Using a sterile scalpel blade, make a controlled
incision through the superficial layer of the skin (FIGURE 43-B), The wound will be
allowed to bleed to ensure adequate vascularity.
• Use sterile gauze or surgical suction to control any bleeding from the wound site. Apply
pressure with a sterile gauze or bandage to stop any bleeding, if necessary.
• For the cream application, a sequential application procedure was employed, wherein
an initial three-day treatment period involved the administration of a chitosan based
anti-inflammatory cream, followed by a subsequent application of a chitosan based
wound healing cream. Apply the cream to the wound site (FIGURE 43-C). Use a sterile
applicator to ensure cleanliness.
• After the procedure, the rabbits were housed in a clean, comfortable environment with
appropriate temperature, humidity, and ventilation, food and water as is also provided.
d) Skin irritation Test
After applying the cream, the wound site is covered with a sterile gauze patch. The first patch
is removed after 5-10 min. If no serious skin reaction is observed, a second patch will be placed.