Figure 30 : Method 02 - alkaline hydrolysis (a) and washing process --------------------- 34
Figure 31 : Method 03- deproteinization process --------------------------------------------- 34
Figure 32: Chitosan hydrochloride production ----------------------------------------------- 37
Figure 33 : Fbc analysis for chitosan (a) and commercial chitosan (b) -------------------- 38
Figure 34:: Wbc analysis for a"chitosan" and b"commercial chitosan"------------------ 39
Figure 35: Biuret reagent ------------------------------------------------------------------------- 39
Figure 36 : Disc diffusion test -------------------------------------------------------------------- 45
Figure 37 : Muller-hinton culture medium ---------------------------------------------------- 46
Figure 38 : Inoculum preparation--------------------------------------------------------------- 47
Figure 39 : Chitosan hydrochloride gel -------------------------------------------------------- 47
Figure 40: Ingredients for cream preparation ------------------------------------------------ 49
Figure 41 : Chitosan hydrochloride gel for cream preparation----------------------------- 50
Figure 42: Albino rabbit (oryctolaguscuniculus) --------------------------------------------- 51
Figure 43: Wounding procedure ---------------------------------------------------------------- 53
Figure 44 : Cream application on wound site ------------------------------------------------- 54
Figure 45: First-degree burn on rabbits skin -------------------------------------------------- 54
Figure 46: 2% chitosan solution ----------------------------------------------------------------- 55
Figure 47: Treatment of paper with chitosan hydrochloride ------------------------------- 55
Figure 48:Chitin (a) and chitosan (b) final aspect -------------------------------------------- 59
Figure 49:Moisture content of chitin and chitosan ------------------------------------------- 60
Figure 50:Protein identification test ------------------------------------------------------------ 62
Figure 51:Chitosan solubility in acetic acid---------------------------------------------------- 63
Figure 52:Chemical reaction for amine group in chitosan with water molecule (ahing
and wid,2016)--------------------------------------------------------------------------------------- 63
Figure 53: FT-IR analyses of chitin sample ---------------------------------------------------- 64
Figure 54:FT-IR analyses of chitosan sample ------------------------------------------------- 65
Figure 55:Determination of the intrinsic viscosity of chitosan ------------------------------ 66
Figure 56: FT-IR analysis of chitosan hydrochloride ---------------------------------------- 68
Figure 57: Antimicrobial activity of chitosan hydrochloride-------------------------------- 70
Figure 58: Cream apparence--------------------------------------------------------------------- 71
Figure 59:Chitosanhydrochloride gel texture ------------------------------------------------- 71
Figure 60:Chitosan films ------------------------------------------------------------------------- 75
Figure 61:Treatment of paper with chitosan hydrochloride -------------------------------- 76
Figure 62 : Product prototype ------------------------------------------------------------------ 101