81
2. For reasons of comparability, all final solutions prepared by
thin-film hydration and organic solvent evaporation protocols
had the same polymer concentration (1x10
−3
g.mL
−1
).
3. In all cases, Milli-Q water was filtered before each dissolution
to ensure its clearance from dust and bacteria.
4. In the case of organic cosolvent protocol, when placing the
vial of filtered Milli-Q water on the hot plate, the stirring
speed should be enough to develop a vortex. This allows for
efficient and rapid mixing of the solutions resulting in more
stable and finely dispersed nanoparticles.
5. After the injection of drug/copolymer solution into the vial
containing the filtered Milli-Q water, the solution mixture
turned turbid due to the mixing of the solvents used and the
formation of drug-loaded micelles.
6. The absorbance of solutions in the UV-Vis range should be
below or equal to 1.0 in order for the Beer-Lambert law to be
applicable. If not, the solutions must be diluted as much as
needed in order for their absorbance to be within this range.
7. Baselines of all ATR-FTIR spectra were corrected after the
measurement by subtracting the baseline in air.
8. From the ATR-FTIR spectrum of Pluronic F-127 with encapsulated curcumin the existence of a peak at 3312 cm
−1
corresponding to O-H stretching is observed (Fig. 4a). This
particular peak is attributed to internal moisture of the
sample.
9. The size distributions obtained from DLS are from measurements at 90°, 25 °C, and pH = 7.
10. It is recommended that syringes should not have Luer Lock in
order to minimize the risk of contamination.
11. It must be noted that the solutions prepared via the thin-film
protocol present better colloidal stability since precipitation
was observed in the solutions prepared by the organic solvent
method after several hours or days depending on the copolymer/drug mixture.
Acknowledgments
This work has been co-financed by the European Union and Greek
national funds through the program “Support for Researchers
with Emphasis on Young Researchers” (call code: EDBM34, ΚΕ
14995) and under the research title “Preparation and study of
innovative forms of administration of pharmaceutical molecules
targeting at improved pharmacological properties.”
Drug Delivery: Hydrophobic Drug Encapsulation into Amphiphilic Block…
2. For reasons of comparability, all final solutions prepared by
thin-film hydration and organic solvent evaporation protocols
had the same polymer concentration (1x10
−3
g.mL
−1
).
3. In all cases, Milli-Q water was filtered before each dissolution
to ensure its clearance from dust and bacteria.
4. In the case of organic cosolvent protocol, when placing the
vial of filtered Milli-Q water on the hot plate, the stirring
speed should be enough to develop a vortex. This allows for
efficient and rapid mixing of the solutions resulting in more
stable and finely dispersed nanoparticles.
5. After the injection of drug/copolymer solution into the vial
containing the filtered Milli-Q water, the solution mixture
turned turbid due to the mixing of the solvents used and the
formation of drug-loaded micelles.
6. The absorbance of solutions in the UV-Vis range should be
below or equal to 1.0 in order for the Beer-Lambert law to be
applicable. If not, the solutions must be diluted as much as
needed in order for their absorbance to be within this range.
7. Baselines of all ATR-FTIR spectra were corrected after the
measurement by subtracting the baseline in air.
8. From the ATR-FTIR spectrum of Pluronic F-127 with encapsulated curcumin the existence of a peak at 3312 cm
−1
corresponding to O-H stretching is observed (Fig. 4a). This
particular peak is attributed to internal moisture of the
sample.
9. The size distributions obtained from DLS are from measurements at 90°, 25 °C, and pH = 7.
10. It is recommended that syringes should not have Luer Lock in
order to minimize the risk of contamination.
11. It must be noted that the solutions prepared via the thin-film
protocol present better colloidal stability since precipitation
was observed in the solutions prepared by the organic solvent
method after several hours or days depending on the copolymer/drug mixture.
Acknowledgments
This work has been co-financed by the European Union and Greek
national funds through the program “Support for Researchers
with Emphasis on Young Researchers” (call code: EDBM34, ΚΕ
14995) and under the research title “Preparation and study of
innovative forms of administration of pharmaceutical molecules
targeting at improved pharmacological properties.”
Drug Delivery: Hydrophobic Drug Encapsulation into Amphiphilic Block…