75
1. Weigh 10 mg PEO-b-PCL in a vial and add 3 mL THF. Wait
for the polymer to dissolve (as inspected visually).
2. Weigh 0.6 mg IND in another vial and add 200 μL THF. Wait
for the drug to dissolve (as inspected visually).
3. Mix IND solution with PEO-b-PCL solution.
4. In a pre-weighed vial add 10 mL filtered Milli-Q H 2 O and a
magnetic stirrer. Put the vial over a hot plate and turn on the
stirring as much as needed in order to see a vortex (see Note 4).
5. Inject fast the PEO-b-PCL/IND mixture in the stirring water
using a syringe and wait until the solution becomes clear
(appx. 5–10 min) (see Note 5).
6. Transfer the solution in a 100 mL round-bottom flask.
7. Place the flask in a rotary evaporator, set the water bath temperature at 40 °C and the stirring at 600 rpm.
8. Leave the flask in the rotary evaporator until the whole amount
of THF is evaporated.
9. When THF is evaporated, remove the flask and put the solution back in the vial.
10. Weigh the solution. The volume must be 10 mL. If it is less, it
means that part of the water was also evaporated during the
evaporation.
11. Fill out with filtered Milli-Q H 2 O till 10 mL final volume.
1. In a glass vial weigh 10 mg PEO-b-PCL and add 3 mL THF.
2. In a second vial, weigh 0.6 mg IND and add 200 μL THF.
3. Mix the PEO-b-PCL solution with the solution of IND.
4. Transfer the solution in a 50 mL round-bottom flask.
5. Place the flask in a rotary evaporator, set the water bath temperature at 40 °C and the stirring at 600 rpm.
6. Leave the flask in the rotary evaporator until the whole amount
of THF is evaporated and a thin film of polymer and IND will
be formed around the inner part of the flask.
7. When the thin film is formed, wait for 20 more minutes before
removing the flask from the evaporator in order to ensure that
there is no THF left.
8. Remove the flask and add 10 mL filtered Milli-Q H 2 O.
9. Gently shake the flask with your hand until the entire thin film
is dissolved.
10. Transfer the solution to a clean vial.
3.3 Solution of PEOb- PCL with 20% IND
Encapsulated (Organic
Solvent Protocol)
3.4 Solutions
of PEO-b-PCL
with 20% IND
Encapsulated
(Thin-Film Protocol)
Drug Delivery: Hydrophobic Drug Encapsulation into Amphiphilic Block…
1. Weigh 10 mg PEO-b-PCL in a vial and add 3 mL THF. Wait
for the polymer to dissolve (as inspected visually).
2. Weigh 0.6 mg IND in another vial and add 200 μL THF. Wait
for the drug to dissolve (as inspected visually).
3. Mix IND solution with PEO-b-PCL solution.
4. In a pre-weighed vial add 10 mL filtered Milli-Q H 2 O and a
magnetic stirrer. Put the vial over a hot plate and turn on the
stirring as much as needed in order to see a vortex (see Note 4).
5. Inject fast the PEO-b-PCL/IND mixture in the stirring water
using a syringe and wait until the solution becomes clear
(appx. 5–10 min) (see Note 5).
6. Transfer the solution in a 100 mL round-bottom flask.
7. Place the flask in a rotary evaporator, set the water bath temperature at 40 °C and the stirring at 600 rpm.
8. Leave the flask in the rotary evaporator until the whole amount
of THF is evaporated.
9. When THF is evaporated, remove the flask and put the solution back in the vial.
10. Weigh the solution. The volume must be 10 mL. If it is less, it
means that part of the water was also evaporated during the
evaporation.
11. Fill out with filtered Milli-Q H 2 O till 10 mL final volume.
1. In a glass vial weigh 10 mg PEO-b-PCL and add 3 mL THF.
2. In a second vial, weigh 0.6 mg IND and add 200 μL THF.
3. Mix the PEO-b-PCL solution with the solution of IND.
4. Transfer the solution in a 50 mL round-bottom flask.
5. Place the flask in a rotary evaporator, set the water bath temperature at 40 °C and the stirring at 600 rpm.
6. Leave the flask in the rotary evaporator until the whole amount
of THF is evaporated and a thin film of polymer and IND will
be formed around the inner part of the flask.
7. When the thin film is formed, wait for 20 more minutes before
removing the flask from the evaporator in order to ensure that
there is no THF left.
8. Remove the flask and add 10 mL filtered Milli-Q H 2 O.
9. Gently shake the flask with your hand until the entire thin film
is dissolved.
10. Transfer the solution to a clean vial.
3.3 Solution of PEOb- PCL with 20% IND
Encapsulated (Organic
Solvent Protocol)
3.4 Solutions
of PEO-b-PCL
with 20% IND
Encapsulated
(Thin-Film Protocol)
Drug Delivery: Hydrophobic Drug Encapsulation into Amphiphilic Block…