74
3. Mix the Pluronic F-127 solution with the curcumin solution
in one vial.
4. In a pre-weighed vial, add 10 mL filtered Milli-Q water and a
magnetic stirrer, place it over a hot plate, and turn on the
stirring as much as needed in order to see a vortex.
5. Inject fast the mixed Pluronic F-127/curcumin acetone solution in the stirred water and wait until the solution becomes
clear (approximately 5–10 min).
6. Transfer the solution in a 100 mL round-bottom flask.
7. Place the flask in a rotary evaporator, set the water bath
temperature at 40 °C and the stirring at 600 rpm.
8. Leave the flask in the rotary evaporator until the whole amount
of acetone is evaporated.
9. When acetone is evaporated, remove the flask and put the
solution back in the vial.
10. Weigh the solution in a pre-weighed vial. The volume must be
10 mL in order to achieve a concentration of 1 × 10
−3
g/mL
(see Note 2). If it is less, it means that part of the water was
also evaporated during the evaporation step.
11. Fill out with filtered Milli-Q H 2 O till 10 mL final volume
accordingly (see Note 3).
1. In a glass vial weigh 10 mg Pluronic F-127 and add 3 mL
acetone. Wait for the polymer to dissolve (as inspected
visually).
2. In a second vial, weigh 1.5 mg curcumin and add 200 μL
acetone. Wait for the drug to dissolve (as inspected visually).
3. Mix the Pluronic F-127 solution with the curcumin solution
in a vial.
4. Transfer the solution in a 50 mL round-bottom flask.
5. Place the flask in a rotary evaporator, set the water bath temperature at 40 °C and the stirring at 600 rpm.
6. Leave the flask in the rotary evaporator until the whole amount
of acetone is evaporated and a thin film of polymer and curcumin will be formed around the inner part of the flask.
7. When the thin film is formed, wait for 20 more minutes before
removing the flask from the rotary evaporator in order to
make sure that there is no acetone left.
8. Remove the flask and add 10 mL filtered Milli-Q H 2 O.
9. Gently shake the flask with your hand until the entire thin film
is dissolved.
10. Transfer the solution to a clean vial.
3.2 Solution of
Pluronic F-127
with 50% Curcumin
Encapsulated
(Thin- Film Protocol)
Angeliki Chroni et al.
3. Mix the Pluronic F-127 solution with the curcumin solution
in one vial.
4. In a pre-weighed vial, add 10 mL filtered Milli-Q water and a
magnetic stirrer, place it over a hot plate, and turn on the
stirring as much as needed in order to see a vortex.
5. Inject fast the mixed Pluronic F-127/curcumin acetone solution in the stirred water and wait until the solution becomes
clear (approximately 5–10 min).
6. Transfer the solution in a 100 mL round-bottom flask.
7. Place the flask in a rotary evaporator, set the water bath
temperature at 40 °C and the stirring at 600 rpm.
8. Leave the flask in the rotary evaporator until the whole amount
of acetone is evaporated.
9. When acetone is evaporated, remove the flask and put the
solution back in the vial.
10. Weigh the solution in a pre-weighed vial. The volume must be
10 mL in order to achieve a concentration of 1 × 10
−3
g/mL
(see Note 2). If it is less, it means that part of the water was
also evaporated during the evaporation step.
11. Fill out with filtered Milli-Q H 2 O till 10 mL final volume
accordingly (see Note 3).
1. In a glass vial weigh 10 mg Pluronic F-127 and add 3 mL
acetone. Wait for the polymer to dissolve (as inspected
visually).
2. In a second vial, weigh 1.5 mg curcumin and add 200 μL
acetone. Wait for the drug to dissolve (as inspected visually).
3. Mix the Pluronic F-127 solution with the curcumin solution
in a vial.
4. Transfer the solution in a 50 mL round-bottom flask.
5. Place the flask in a rotary evaporator, set the water bath temperature at 40 °C and the stirring at 600 rpm.
6. Leave the flask in the rotary evaporator until the whole amount
of acetone is evaporated and a thin film of polymer and curcumin will be formed around the inner part of the flask.
7. When the thin film is formed, wait for 20 more minutes before
removing the flask from the rotary evaporator in order to
make sure that there is no acetone left.
8. Remove the flask and add 10 mL filtered Milli-Q H 2 O.
9. Gently shake the flask with your hand until the entire thin film
is dissolved.
10. Transfer the solution to a clean vial.
3.2 Solution of
Pluronic F-127
with 50% Curcumin
Encapsulated
(Thin- Film Protocol)
Angeliki Chroni et al.