230
PNIPAM- b-PLA 2, respectively. Polydispersity index (M w /M n )
of the copolymers was 1.55 and 1.16, respectively.
3. PLA is a hydrophobic polymer, while PNIPAM is hydrophilic
below and becomes hydrophobic above the LCST value [6,
15].
4. For DSC analysis, dry bilayers that were hydrated with low
amount of aqueous medium were utilized, resulting in MLV
formation, in order to have high concentration and strong
DSC signal.
5. Each ratio of 9:0.02, 9:0.05, 9:0.1, 9:0.2, 9:0.5, and 9:1
stands for phospholipid:copolymer molar ratio.
6. The PBS medium simulates the pH, osmolarity, and ion concentrations of the human physiological environment.
7. For liposomal development, the dry lipid films were hydrated
with more amount of aqueous medium, which resulted in the
proper concentration for liposomes to self-assemble and provide SUVs after size reduction.
8. Phospholipids and the particular diblock copolymer are soluble in chloroform [16].
9. Most of the organic solvent evaporates rapidly under these
conditions; however, the formed dry films are maintained as
such for a short period of time.
10. The bilayers are considered as fully hydrated and the concentration of the final system is 150 mg mL
−1
.
11. Equilibration is necessary for samples that have been previously hydrated with aqueous media.
12. The isotherm at the analysis starting point is necessary to
ensure that the sample is at equilibrium. For a lyotropic liquid
crystalline system like lipidic membranes, it is very important
to ensure that it is in equilibrium state before analysis.
13. The analysis temperature range should include all membrane
thermodynamic phenomena of interest that might be affected
by the insertion of the polymers. In addition, the LCST of the
PNIPAM was taken into account.
14. Three heating scans are necessary to ensure the reproducibility
of the thermodynamic phenomena, as well as the equilibrium
of the studied system.
15. 5 °C min
−1
is a generally accepted heating or cooling rate for
lipidic bilayers [11].
16. Constant pressure is essential in this type of calorimetry, in
order to monitor the heat capacity of the sample under constant pressure (C p ).
Nikolaos Naziris et al.
PNIPAM- b-PLA 2, respectively. Polydispersity index (M w /M n )
of the copolymers was 1.55 and 1.16, respectively.
3. PLA is a hydrophobic polymer, while PNIPAM is hydrophilic
below and becomes hydrophobic above the LCST value [6,
15].
4. For DSC analysis, dry bilayers that were hydrated with low
amount of aqueous medium were utilized, resulting in MLV
formation, in order to have high concentration and strong
DSC signal.
5. Each ratio of 9:0.02, 9:0.05, 9:0.1, 9:0.2, 9:0.5, and 9:1
stands for phospholipid:copolymer molar ratio.
6. The PBS medium simulates the pH, osmolarity, and ion concentrations of the human physiological environment.
7. For liposomal development, the dry lipid films were hydrated
with more amount of aqueous medium, which resulted in the
proper concentration for liposomes to self-assemble and provide SUVs after size reduction.
8. Phospholipids and the particular diblock copolymer are soluble in chloroform [16].
9. Most of the organic solvent evaporates rapidly under these
conditions; however, the formed dry films are maintained as
such for a short period of time.
10. The bilayers are considered as fully hydrated and the concentration of the final system is 150 mg mL
−1
.
11. Equilibration is necessary for samples that have been previously hydrated with aqueous media.
12. The isotherm at the analysis starting point is necessary to
ensure that the sample is at equilibrium. For a lyotropic liquid
crystalline system like lipidic membranes, it is very important
to ensure that it is in equilibrium state before analysis.
13. The analysis temperature range should include all membrane
thermodynamic phenomena of interest that might be affected
by the insertion of the polymers. In addition, the LCST of the
PNIPAM was taken into account.
14. Three heating scans are necessary to ensure the reproducibility
of the thermodynamic phenomena, as well as the equilibrium
of the studied system.
15. 5 °C min
−1
is a generally accepted heating or cooling rate for
lipidic bilayers [11].
16. Constant pressure is essential in this type of calorimetry, in
order to monitor the heat capacity of the sample under constant pressure (C p ).
Nikolaos Naziris et al.