224
1. Polystyrene cuvettes (4.5 mL).
2. Liposomal samples/small unilamellar vesicles (SUVs) (see Note
7):
(a) DPPC.
(b) DPPC:PNIPAM-b-PLA 1 9:0.02.
(c) DPPC:PNIPAM-b-PLA 1 9:0.05.
(d) DPPC:PNIPAM-b-PLA 1 9:0.1.
3. HPLC-grade H 2 O.
3 Methods
The methods described herein are a conventional evaporation
technique to prepare thermoresponsive chimeric bilayers, DSC
sample preparation, sample analysis, extraction and analysis of the
results, thin-film hydration method for developing thermoresponsive chimeric liposomes or liposomes in general, physicochemical
characterization of the latter through dynamic light scattering, and
a simple heating protocol to test the thermoresponsive behavior of
the developed nanoparticles. The results from the DSC analysis
and the physicochemical characterization are presented and
discussed.
1. Dissolve the desired amount of DPPC and each one of the chimeric nanosystems DPPC:PNIPAM-b-PLA 1 or 2 at 9:0.02,
9:0.05, 9:0.1, 9:0.2, 9:0.5, and 9:1 molar ratios in chloroform
(see Note 8).
2. Evaporate the solvent under vacuum and heat conditions
(−1 bar and 40 °C), using a rotary evaporator.
3. Maintain the formed dry lipid films under these conditions for
30 min (see Note 9).
4. Place the dry lipid films in a desiccator, for at least 24 h, in order
to remove possible traces of solvent.
1. Prepare each sample for DSC analysis by weighting 3 mg of dry
powder of lipidic or chimeric nanosystem inside a 40 μL aluminum crucible and hydrating it with 20 μL PBS medium (see
Notes 10).
2. Seal each crucible by using a sealing press, in order to make a
hermetic pan, and leave it to rest for a 15-min period, in order
to achieve equilibration of the sample (see Note 11).
2.4 Dynamic Light
Scattering (DLS)
and Heating Protocol
3.1 Preparation
of Thermoresponsive
Chimeric Bilayers
3.2 DSC Sample
Preparation
Nikolaos Naziris et al.
1. Polystyrene cuvettes (4.5 mL).
2. Liposomal samples/small unilamellar vesicles (SUVs) (see Note
7):
(a) DPPC.
(b) DPPC:PNIPAM-b-PLA 1 9:0.02.
(c) DPPC:PNIPAM-b-PLA 1 9:0.05.
(d) DPPC:PNIPAM-b-PLA 1 9:0.1.
3. HPLC-grade H 2 O.
3 Methods
The methods described herein are a conventional evaporation
technique to prepare thermoresponsive chimeric bilayers, DSC
sample preparation, sample analysis, extraction and analysis of the
results, thin-film hydration method for developing thermoresponsive chimeric liposomes or liposomes in general, physicochemical
characterization of the latter through dynamic light scattering, and
a simple heating protocol to test the thermoresponsive behavior of
the developed nanoparticles. The results from the DSC analysis
and the physicochemical characterization are presented and
discussed.
1. Dissolve the desired amount of DPPC and each one of the chimeric nanosystems DPPC:PNIPAM-b-PLA 1 or 2 at 9:0.02,
9:0.05, 9:0.1, 9:0.2, 9:0.5, and 9:1 molar ratios in chloroform
(see Note 8).
2. Evaporate the solvent under vacuum and heat conditions
(−1 bar and 40 °C), using a rotary evaporator.
3. Maintain the formed dry lipid films under these conditions for
30 min (see Note 9).
4. Place the dry lipid films in a desiccator, for at least 24 h, in order
to remove possible traces of solvent.
1. Prepare each sample for DSC analysis by weighting 3 mg of dry
powder of lipidic or chimeric nanosystem inside a 40 μL aluminum crucible and hydrating it with 20 μL PBS medium (see
Notes 10).
2. Seal each crucible by using a sealing press, in order to make a
hermetic pan, and leave it to rest for a 15-min period, in order
to achieve equilibration of the sample (see Note 11).
2.4 Dynamic Light
Scattering (DLS)
and Heating Protocol
3.1 Preparation
of Thermoresponsive
Chimeric Bilayers
3.2 DSC Sample
Preparation
Nikolaos Naziris et al.