223
2. PNIPAM-b-PLA 1 (66–34% w/w, M w   =  18,000) (see Notes
1–3) (Fig. 1B).
3. PNIPAM-b-PLA 2 (50–50% w/w, M w  = 6,400).
4. Chloroform.
1. DSC aluminum pans (40 μL).
2. Pure indium (T m  = 156.6 °C).
3. Bilayer samples/multilamellar vesicles (MLVs) (see Note 4):
(a) DPPC.
(b) DPPC:PNIPAM-b-PLA 1 9:0.02 (see Note 5).
(c) DPPC:PNIPAM-b-PLA 1 9:0.05.
(d) DPPC:PNIPAM-b-PLA 1 9:0.1.
(e) DPPC:PNIPAM-b-PLA 1 9:0.2.
(f) DPPC:PNIPAM-b-PLA 1 9:0.5.
(g) DPPC:PNIPAM-b-PLA 1 9:1.
(h) PNIPAM-b-PLA 1.
(i) DPPC:PNIPAM-b-PLA 2 9:0.02.
(j) DPPC:PNIPAM-b-PLA 2 9:0.05.
(k) DPPC:PNIPAM-b-PLA 2 9:0.1.
(l) DPPC:PNIPAM-b-PLA 2 9:0.2.
(m)
DPPC:PNIPAM-b-PLA 2 9:0.5.
(n) DPPC:PNIPAM-b-PLA 2 9:1.
(o) PNIPAM-b-PLA 2.
4. PBS medium (pH = 7.4) (see Note 6).
1. DPPC.
2. PNIPAM-b-PLA 1.
3. Chloroform.
4. PBS medium (pH = 7.4).
2.2 Differential
Scanning Calorimetry
(DSC)
2.3 Preparation
of Thermoresponsive
Chimeric Liposomes
Fig. 1 Molecular structures of +++ (A) DPPC and (B) PNIPAM-b-PLA 1 (with n:m weight ratio 34:64) or 2 (with
n:m weight ratio 50:50)
Thermodynamics and Functionality of Thermoresponsive Chimeric Nanosystems