213
8. Record the absorption measurements after each interval time
at a wavelength of 337 nm (see Note 6).
1. Clean the working place in the cabinet with alcohol every time
to prevent contamination.
2. Place sterilized bottles and laboratory bottle-top filter under
hood.
3. Measure out the final volume required (900 mL) of pure water
into the 1000 mL beaker.
3.6 Preparation
of DMEM Culture
Media for Cell Line
0,000
0,005
0,010
0,015
0,020
0,025
0,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
Absorbance
Concentration (mg/mL)
Fig. 8 PREC-2 NPs showing calibration curve (abs vs. conc.) obtained by UV/visible spectrophotometer
0
100
200
300
4 00
0
2
4
6
8
10
12
Drug Release (%)
t (hour)
Fig. 9 Release profiles of ceranib-2 from PRE nanoparticles
Antitumor Efficacy of Ceranib-2 with Nano-Formulation of PEG and Rosin Esters
8. Record the absorption measurements after each interval time
at a wavelength of 337 nm (see Note 6).
1. Clean the working place in the cabinet with alcohol every time
to prevent contamination.
2. Place sterilized bottles and laboratory bottle-top filter under
hood.
3. Measure out the final volume required (900 mL) of pure water
into the 1000 mL beaker.
3.6 Preparation
of DMEM Culture
Media for Cell Line
0,000
0,005
0,010
0,015
0,020
0,025
0,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
Absorbance
Concentration (mg/mL)
Fig. 8 PREC-2 NPs showing calibration curve (abs vs. conc.) obtained by UV/visible spectrophotometer
0
100
200
300
4 00
0
2
4
6
8
10
12
Drug Release (%)
t (hour)
Fig. 9 Release profiles of ceranib-2 from PRE nanoparticles
Antitumor Efficacy of Ceranib-2 with Nano-Formulation of PEG and Rosin Esters