196
10. Generally, a range of 2–80 °C can be applied in the calorimeter. This feature is convenient for the estimation of the heat
capacity (ΔC p ) of a cyclodextrin-guest system as it can be
achieved by conducting the same experiment under different
temperature conditions.
11. For an unknown interaction, a DP equal to 5 or 6 μcal s
−1
is
recommended. However, if the titration curves cross the measurement range of the instrument, the user can change the DP
value.
12. A small number of injections of 10–20 is ample to derive reliable results. More injections do not necessarily lead to more
accurate results [24].
13. The time spacing between the injections offers time to the
instrument to calibrate the ΔΤ between the sample and the
reference cell so the signal will return back to the baseline
(DP).
14. The endothermic process observed upon the titration of losartan is due to the fact that the drug forms aggregates which are
separated upon dilution into the sample cell’s content [39].
15. During the first titration, the heat measurement may not be
accurate. Thus, the initial titration is pilot, injecting a smaller
volume of the ligand into the sample cell compared to the rest
titrations (in order to not waste sample) and is excluded from
the data analysis.
16. If the stoichiometry of an interaction is known, the one set of
site model may be directly applied. In other case, additional
fitting models are provided like the two sets of site model, the
competitive binding, and the dissociation model.
Acknowledgments
This work has been co-financed by the European Union and Greek
national funds through the program “Support for Researchers
with Emphasis on Young Researchers” (call code: EDBM34, ΚΕ
14995) and under the research title “Preparation and study of
innovative forms of administration of pharmaceutical molecules
targeting at improved pharmacological properties.”
References
1. EMA (2017) Cyclodextrins used as excipients. Accessed 11 July, 2019. https://
w w w. e m a . e u r o p a . e u / e n / d o c u m e n t s /
cyclodextrins-used-excipients-medicinal-products-human-use_en.pdf
2. Fenyvesi E, Vikmon M, Szente L (2016)
Cyclodextrins in food technology and human
nutrition: benefits and limitations. Crit Rev
Maria V. Chatziathanasiadou et al.
10. Generally, a range of 2–80 °C can be applied in the calorimeter. This feature is convenient for the estimation of the heat
capacity (ΔC p ) of a cyclodextrin-guest system as it can be
achieved by conducting the same experiment under different
temperature conditions.
11. For an unknown interaction, a DP equal to 5 or 6 μcal s
−1
is
recommended. However, if the titration curves cross the measurement range of the instrument, the user can change the DP
value.
12. A small number of injections of 10–20 is ample to derive reliable results. More injections do not necessarily lead to more
accurate results [24].
13. The time spacing between the injections offers time to the
instrument to calibrate the ΔΤ between the sample and the
reference cell so the signal will return back to the baseline
(DP).
14. The endothermic process observed upon the titration of losartan is due to the fact that the drug forms aggregates which are
separated upon dilution into the sample cell’s content [39].
15. During the first titration, the heat measurement may not be
accurate. Thus, the initial titration is pilot, injecting a smaller
volume of the ligand into the sample cell compared to the rest
titrations (in order to not waste sample) and is excluded from
the data analysis.
16. If the stoichiometry of an interaction is known, the one set of
site model may be directly applied. In other case, additional
fitting models are provided like the two sets of site model, the
competitive binding, and the dissociation model.
Acknowledgments
This work has been co-financed by the European Union and Greek
national funds through the program “Support for Researchers
with Emphasis on Young Researchers” (call code: EDBM34, ΚΕ
14995) and under the research title “Preparation and study of
innovative forms of administration of pharmaceutical molecules
targeting at improved pharmacological properties.”
References
1. EMA (2017) Cyclodextrins used as excipients. Accessed 11 July, 2019. https://
w w w. e m a . e u r o p a . e u / e n / d o c u m e n t s /
cyclodextrins-used-excipients-medicinal-products-human-use_en.pdf
2. Fenyvesi E, Vikmon M, Szente L (2016)
Cyclodextrins in food technology and human
nutrition: benefits and limitations. Crit Rev
Maria V. Chatziathanasiadou et al.