105
ROE data is shown in Fig. 5. The most critical ROE observed is
between the protons 5 and 2′ which defines the relative orientation
of the ring with respect to the chain of the major conformer.
The gradual addition of 5-DSA in the D 2 O/CΗ 3 ΟD/SDS-d 25
sample is shown in Fig. 6. There are two major characteristics of
the spectra obtained after the four subsequent additions of DSA.
(a) The chemical shifts due to the captopril are not affected and (b)
all the peaks decrease in intensity and are broadened which results
in losing the multiplicity information. For example, after the second addition of DSA, the peaks resonated at 1.21 ppm and
1.19 ppm merge only to the one at 1.21 ppm. Thus, the information for the minor conformer depicted at 1.19 ppm is lost. Clearly,
the results show that 5-DSA affects significantly all peaks of captopril. This means that DSA which is localized in intermediate polarity region (interface) of SDS is in spatial vicinity with captopril.
Thus, captopril is localized in the interface of SDS. In Fig. 7, a
cartoon is presented in which are shown the interactions of captopril with SDS-d 25 micelles.
5 Notes
1. During the sonication heat is generated, so the sample should
be in an ice bath; otherwise there is a danger of breaking the
glass vial.
2. Spectral precaution of the sonicator tip is given in order not to
touch the bottom or sides of the vial.
Fig. 5 This model satisfies the two critical ROEs 2′-5 and 2–5. The average distances of these two critical ROEs are 2.49 Å and 3.82 Å
Drug Incorporation in Micelles
ROE data is shown in Fig. 5. The most critical ROE observed is
between the protons 5 and 2′ which defines the relative orientation
of the ring with respect to the chain of the major conformer.
The gradual addition of 5-DSA in the D 2 O/CΗ 3 ΟD/SDS-d 25
sample is shown in Fig. 6. There are two major characteristics of
the spectra obtained after the four subsequent additions of DSA.
(a) The chemical shifts due to the captopril are not affected and (b)
all the peaks decrease in intensity and are broadened which results
in losing the multiplicity information. For example, after the second addition of DSA, the peaks resonated at 1.21 ppm and
1.19 ppm merge only to the one at 1.21 ppm. Thus, the information for the minor conformer depicted at 1.19 ppm is lost. Clearly,
the results show that 5-DSA affects significantly all peaks of captopril. This means that DSA which is localized in intermediate polarity region (interface) of SDS is in spatial vicinity with captopril.
Thus, captopril is localized in the interface of SDS. In Fig. 7, a
cartoon is presented in which are shown the interactions of captopril with SDS-d 25 micelles.
5 Notes
1. During the sonication heat is generated, so the sample should
be in an ice bath; otherwise there is a danger of breaking the
glass vial.
2. Spectral precaution of the sonicator tip is given in order not to
touch the bottom or sides of the vial.
Fig. 5 This model satisfies the two critical ROEs 2′-5 and 2–5. The average distances of these two critical ROEs are 2.49 Å and 3.82 Å
Drug Incorporation in Micelles