96
4 Notes
1. Standard curve preparation: Measure the absorbance of the
desired drug in different concentrations at its characteristic
concentration- dependent absorption wavelength. Then plot
the absorbance values and create a linear equation. This equation will be used to determine the concentration of the loaded
and released amount of drug during the treatment. Standard
curve should be prepared for all tested pH values.
2. The vials commonly used in loading and release studies are
polypropylene Eppendorf or Falcon tubes depending on the
sample volume being used.
3. There are many different types of magnetic hyperthermia
apparatus commercially available. In general, they consist of a
coil—or a set of coils of different dimensions—connected to a
monitor that allows changing the value of the applied magnetic field and/or the frequency. The sample is placed in the
center of the coil to ensure homogeneous magnetization of
the sample. The temperature is monitored and recorded
through various apparatus like electronic thermometers, fiberoptic temperature detectors, and thermal cameras.
4. In order to determine the number of alive cells in the suspension and be able to seed the appropriate population required
to perform any of the biological assays, alive cells should be
counted with a hematocytometer after treatment with trypan
blue. It is a standard procedure which has already been published by Springer Protocols [25].
5. The absorption measurement and recording of the MTT plate
can be in the range of 500–600 nm depending on the solvent
and reference filter should be over 600 nm. Control wells
should give values close to zero (± 0.1).
6. Treatment with biocompatible NCs shows higher absorption
values indicating increased viability whereas drug-loaded NCs
show lower absorption values indicating decreased viability.
Acknowledgments
This work has been co-financed by the European Union and Greek
national funds through the program “Support for Researchers
with Emphasis on Young Researchers” (call code: EDBM34, ΚΕ
14995) and under the research title “Preparation and study of
innovative forms of administration of pharmaceutical molecules
targeting at improved pharmacological properties.”
Maria Theodosiou et al.
4 Notes
1. Standard curve preparation: Measure the absorbance of the
desired drug in different concentrations at its characteristic
concentration- dependent absorption wavelength. Then plot
the absorbance values and create a linear equation. This equation will be used to determine the concentration of the loaded
and released amount of drug during the treatment. Standard
curve should be prepared for all tested pH values.
2. The vials commonly used in loading and release studies are
polypropylene Eppendorf or Falcon tubes depending on the
sample volume being used.
3. There are many different types of magnetic hyperthermia
apparatus commercially available. In general, they consist of a
coil—or a set of coils of different dimensions—connected to a
monitor that allows changing the value of the applied magnetic field and/or the frequency. The sample is placed in the
center of the coil to ensure homogeneous magnetization of
the sample. The temperature is monitored and recorded
through various apparatus like electronic thermometers, fiberoptic temperature detectors, and thermal cameras.
4. In order to determine the number of alive cells in the suspension and be able to seed the appropriate population required
to perform any of the biological assays, alive cells should be
counted with a hematocytometer after treatment with trypan
blue. It is a standard procedure which has already been published by Springer Protocols [25].
5. The absorption measurement and recording of the MTT plate
can be in the range of 500–600 nm depending on the solvent
and reference filter should be over 600 nm. Control wells
should give values close to zero (± 0.1).
6. Treatment with biocompatible NCs shows higher absorption
values indicating increased viability whereas drug-loaded NCs
show lower absorption values indicating decreased viability.
Acknowledgments
This work has been co-financed by the European Union and Greek
national funds through the program “Support for Researchers
with Emphasis on Young Researchers” (call code: EDBM34, ΚΕ
14995) and under the research title “Preparation and study of
innovative forms of administration of pharmaceutical molecules
targeting at improved pharmacological properties.”
Maria Theodosiou et al.