290
R. Halim
Table 12.3
(continued)
Study
Species
Biomass state
Pretreatment and cell
rupture
Extraction solvents and
conditions
Maximum
yield/recovery at
optimum conditions
Key findings
Denery et al.
(2004)
H. pluvialis
Red cyst,
freeze-dried
n/a
Pressurised solvent
extraction with
acetone, ethanol,
acetone:ethanol (7:3
v/v), acetone:methanol
(7:3 v/v) or methylene
chloride:methanol (1:3
v/v). pressure
= 1500
psi. temperature
=
40 °C
10.9 mg astaxanthin/g
biomass with
methylene
chloride:methanol (1:3
v/v)
Acetone extraction
obtained the highest
total pigment yield
(47.7 mg pigment/g
biomass).
Increasing extraction
temperature resulted
in pigment
degradation and
decreased total
pigment yield