160
A. Pérez-Gálvez and J. Fontecha
Table 7.1
(continued)
Sample type
Targeted
pigments for
analysis
Stationary phase
Mobile phase
Detection/Identification References
Phytoplankton
monocultures
Carotenoids
and
chlorophylls
C
16 -Amide-5
μm,150 ×
4.6 mm
Gradient; A: methanol; B: acetonitrile;
C: 0.5 M aqueous ammonium acetate;
1 mL/min
UV/Vis: 440,
450 nm—coupled to
fluorescence: ex.,
440 nm, em., 660 nm
Jayaraman
et al. (2011)
Chlorococcum humicola
Carotenoids C
18 -5
μm, 250
× 4.6 mm
Isocratic; 20:70:10
dichloromethane:acetonitrile:methanol
(v/v/v)
UV/Vis:
450 nm—coupled to Q
and APCI source
Sivathanu and
Palaniswamy
(2012)
Dunaliella salina
Carotenoids
and
chlorophylls
C
18 -1.8
μm, 150
× 2.1 mm
Gradient; A: 70:20:10
acetonitrile:methanol:tert-butyl-methyl
ether; B: 10 mM aqueous ammonium
acetate; 0.5 mL/min
UV/Vis:
450 nm—coupled to
Q-TOF and ESI
interface
Fu et al.
(2012)
Isochrysis
spp.
Carotenoids C
30 -5
μm, 250
× 3 mm
Gradient; A: methanol; B: 1:3
isopropanol:hexane (v/v); 0.5 mL/min
UV/Vis:
447 nm—coupled to
ion trap and ESI
interface
Crupi et al.
(2013)
Scenedesmus protuberans
Carotenoids C
30 -5
μm, 250
× 4.6 mm
Gradient; A: methanol; B:
methyl-tert-butyl ether; C: water;
1 mL/min
UV/Vis:
450 nm—coupled to
Q b
-ion trap and APCI
source
Erdogan et al.
(2015)
Porphyridium purpureum
Carotenoids
and
chlorophylls
C
18 -1.7
μm, 50
× 2.1 mm
Gradient; A: 0.01% formic acid in
water; B: 0.01% formic acid in
methanol; 0.4 mL/min
Q-TOF c
and ESI d
interface
Juin et al.
(2015)
(continued)