6 Analytical Protocols in Chlorophyll Analysis
147
Maroneze, M. M., Zepka, L. Q., Lopes, E. J., Pérez-Gálvez, A., & Roca, M. (2019). Chlorophyll
oxidative metabolism during the phototrophic and heterotrophic growth of scenedesmus obliquus.
Antioxidants 8(12), 600.
Montero, O., Macías-Sánchez, M. D., Lama, C. M., Lubián, L. M., Mantell, C., Rodríguez, M., et al.
(2005). Supercritical CO 2 extraction of β-carotene from a marine strain of the cyanobacterium
Synechococcus species. Journal of Agricultural and Food Chemistry, 53, 9701–9707.
Neveux, J. (1988). Extraction of chlorophylls from marine phytoplankton. Verhandlungen des
Internationalen Verein Limnologie, 23, 928–932.
Neveux, J., Seppälä, J., & Dandonneau, Y. (2011). Multivariate analysis of extracted pigments
using spectrophotometric and spectrofluorometric methods. In S. Roy, C. A. Llewellyn, E. S.
Egeland, & G. Johnsen (Eds.), Phytoplankton pigments: Characterization, chemotaxonomy and
applications in oceanography (pp. 343–372). New York: Cambridge University Press.
Nobre, B. P., Villalobos, F., Barragán, B. E., Oliveira, A. C., Batista, A. P., Marques, P. A. S. S.,
et al. (2013). A biorefinery from Nannochloropsis sp. microalga—Extraction of oils and pigments.
Production of biohydrogen from the leftover biomass. Bioresource Technology, 135, 128–136.
Ota, M., Watanabe, H., Kato, Y., Watanabe, M., Sato, Y., Smith, R. L., et al. (2009). Carotenoid
production from Chlorococcum littorale in photoautotrophic cultures with downstream supercritical fluid processing. Journal of Separation Science, 32, 2327–2335.
Pasquet, V., Chérouvrier, J. R., Farhat, F., Thiéry, V., Piot, J. M., Bérard, J. B., et al. (2011). Study
on the microalgal pigments extraction process: Performance of microwave assisted extraction.
Process Biochemistry, 46, 59–67.
Pennington, F. C., Strain, H. H., Svec, W. A., & Katz, J. J. (1964). Preparation and properties of
pyrochlorophyll a, methyl pyrochlorophyllide a, pyropheophytin a, and methylpyropheophorbide
a derived from chlorophyll by decarbomethoxylation. Journal of the American Chemical Society,
86, 1418–1426.
Porra, R. J., Thompson, W. A., & Kreidemann, P. E. (1989). Determination of accurate extinction
coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four
different solvents: Verification of the concentration of chlorophyll standards by atomic absorption
spectrometry. Biochimica et Biophysica Acta, 975, 384–394.
Porra, R. J. (2006). Spectrometric assays for plant, algal and bacterial chlorophyllsin. In B. Grimm,
R. J. Porra, W. Rüdiger, & H. Scheer 542 (Eds.), Chlorophylls and bacteriochlorophylls (pp. 95107). Springer: The Netherlands.
Richards, F. A., & Thompson, T. G. (1952). The estimation and characterisation of plankton populations by pigment analysis. II. A spectrophotometric method for the estimation of plankton
pigments. Journal of Marine Research, 11, 156–172.
Ritchie, R. J. (2006). Consistent sets of spectrophotometric chlorophyll equations for acetone,
methanol and ethanol solvents. Photosynthesis Research, 89, 27–41.
Rowan, K. S. (1989). Photosynthetic pigments of algae. New York: Cambridge University Press.
Roy, S., Wright, S. W., & Jeffrey, S. W. (2011a). Phytoplankton cultures for standard pigments and
their suppliers. In S. Roy, C. A. Llewellyn, E. S. Egeland, & G. Johnsen (Eds.), Phytoplankton
pigments: Characterization, chemotaxonomy and applications in oceanography (pp. 653–657).
New York: Cambridge University Press.
Roy, S., Llewellyn, C. A., Egeland, E. S., & Johnsen, G. (2011b). Phytoplankton pigments: Characterization, chemotaxonomy and applications in oceanography. New York: Cambridge University
Press.
Santoyo, S., Plaza, M., Jaime, L., Ibañez, E., Reglero, G., & Señorans, F. J. (2010). Pressurized
liquid extraction as an alternative process to obtain antiviral agents from the edible microalga
Chlorella vulgaris. Journal of Agricultural and Food Chemistry, 58, 8522–8527.
Sanz, N., Garcia-Blanco, A., Gavalás-Olea, A., Loures, P., & Garrido, J. L. (2015). Phytoplankton
pigment biomarkers: HPLC separation using a pentafluorophenyloctadecyl silica column. Meth.
Ecol. Evol., 6, 1199–1209.
Sartory, D. P., & Grobbelaas, J. U. (1984). Extraction of chlorophyll a from freshwater phytoplankton
for spectrophotometric analysis. Hydrobiologia, 114, 177–187.
147
Maroneze, M. M., Zepka, L. Q., Lopes, E. J., Pérez-Gálvez, A., & Roca, M. (2019). Chlorophyll
oxidative metabolism during the phototrophic and heterotrophic growth of scenedesmus obliquus.
Antioxidants 8(12), 600.
Montero, O., Macías-Sánchez, M. D., Lama, C. M., Lubián, L. M., Mantell, C., Rodríguez, M., et al.
(2005). Supercritical CO 2 extraction of β-carotene from a marine strain of the cyanobacterium
Synechococcus species. Journal of Agricultural and Food Chemistry, 53, 9701–9707.
Neveux, J. (1988). Extraction of chlorophylls from marine phytoplankton. Verhandlungen des
Internationalen Verein Limnologie, 23, 928–932.
Neveux, J., Seppälä, J., & Dandonneau, Y. (2011). Multivariate analysis of extracted pigments
using spectrophotometric and spectrofluorometric methods. In S. Roy, C. A. Llewellyn, E. S.
Egeland, & G. Johnsen (Eds.), Phytoplankton pigments: Characterization, chemotaxonomy and
applications in oceanography (pp. 343–372). New York: Cambridge University Press.
Nobre, B. P., Villalobos, F., Barragán, B. E., Oliveira, A. C., Batista, A. P., Marques, P. A. S. S.,
et al. (2013). A biorefinery from Nannochloropsis sp. microalga—Extraction of oils and pigments.
Production of biohydrogen from the leftover biomass. Bioresource Technology, 135, 128–136.
Ota, M., Watanabe, H., Kato, Y., Watanabe, M., Sato, Y., Smith, R. L., et al. (2009). Carotenoid
production from Chlorococcum littorale in photoautotrophic cultures with downstream supercritical fluid processing. Journal of Separation Science, 32, 2327–2335.
Pasquet, V., Chérouvrier, J. R., Farhat, F., Thiéry, V., Piot, J. M., Bérard, J. B., et al. (2011). Study
on the microalgal pigments extraction process: Performance of microwave assisted extraction.
Process Biochemistry, 46, 59–67.
Pennington, F. C., Strain, H. H., Svec, W. A., & Katz, J. J. (1964). Preparation and properties of
pyrochlorophyll a, methyl pyrochlorophyllide a, pyropheophytin a, and methylpyropheophorbide
a derived from chlorophyll by decarbomethoxylation. Journal of the American Chemical Society,
86, 1418–1426.
Porra, R. J., Thompson, W. A., & Kreidemann, P. E. (1989). Determination of accurate extinction
coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four
different solvents: Verification of the concentration of chlorophyll standards by atomic absorption
spectrometry. Biochimica et Biophysica Acta, 975, 384–394.
Porra, R. J. (2006). Spectrometric assays for plant, algal and bacterial chlorophyllsin. In B. Grimm,
R. J. Porra, W. Rüdiger, & H. Scheer 542 (Eds.), Chlorophylls and bacteriochlorophylls (pp. 95107). Springer: The Netherlands.
Richards, F. A., & Thompson, T. G. (1952). The estimation and characterisation of plankton populations by pigment analysis. II. A spectrophotometric method for the estimation of plankton
pigments. Journal of Marine Research, 11, 156–172.
Ritchie, R. J. (2006). Consistent sets of spectrophotometric chlorophyll equations for acetone,
methanol and ethanol solvents. Photosynthesis Research, 89, 27–41.
Rowan, K. S. (1989). Photosynthetic pigments of algae. New York: Cambridge University Press.
Roy, S., Wright, S. W., & Jeffrey, S. W. (2011a). Phytoplankton cultures for standard pigments and
their suppliers. In S. Roy, C. A. Llewellyn, E. S. Egeland, & G. Johnsen (Eds.), Phytoplankton
pigments: Characterization, chemotaxonomy and applications in oceanography (pp. 653–657).
New York: Cambridge University Press.
Roy, S., Llewellyn, C. A., Egeland, E. S., & Johnsen, G. (2011b). Phytoplankton pigments: Characterization, chemotaxonomy and applications in oceanography. New York: Cambridge University
Press.
Santoyo, S., Plaza, M., Jaime, L., Ibañez, E., Reglero, G., & Señorans, F. J. (2010). Pressurized
liquid extraction as an alternative process to obtain antiviral agents from the edible microalga
Chlorella vulgaris. Journal of Agricultural and Food Chemistry, 58, 8522–8527.
Sanz, N., Garcia-Blanco, A., Gavalás-Olea, A., Loures, P., & Garrido, J. L. (2015). Phytoplankton
pigment biomarkers: HPLC separation using a pentafluorophenyloctadecyl silica column. Meth.
Ecol. Evol., 6, 1199–1209.
Sartory, D. P., & Grobbelaas, J. U. (1984). Extraction of chlorophyll a from freshwater phytoplankton
for spectrophotometric analysis. Hydrobiologia, 114, 177–187.