6 Analytical Protocols in Chlorophyll Analysis
145
Acknowledgements This work was supported by the Comisión Interministerial de Ciencia y
Tecnología (CICYT-EU, Spanish and European Government, grant number RTI2018-095415-BI00).
References
Airs, R. L. (2018). Mass spectrometry of chlorophylls from phototrophic prokaryotes. Current
Organic Chemistry, 22, 877–884.
Airs, R., & Garrido, J. L. (2011). Liquid chromatography-mass spectrometry for pigment analysis. In S. Roy, C. A. Llewellyn, E. S. Egeland, & G. Johnsen (Eds.), Phytoplankton pigments:
Characterization, chemotaxonomy and applications in oceanography (pp. 314–342). New York:
Cambridge University Press.
Airs, R. L., Temperton, B., Sambles, B., Farnham, G., Skill, S. C., & Llewellyn, C. A. (2014).
Chlorophyll f and chlorophyll d are produced in the cyanobacterium Chlorogloeopsis fritschii
when cultured under natural light and near-infrared radiation. FEBS Letters, 588, 3770–3777.
Arnon, D. I. (1949). Copper enzymes in isolated chloroplasts: Polyphenoloxidase in Beta vulgaris.
Plant Physiology, 24, 1–15.
Barrett, J., & Jeffries, S. W. (1971). A note on the occurrence of chlorophyllase in marine. Journal
of Experimental Marine Biology and Ecology, 7, 255–262.
Bowles, N. D., Paerl, H. W., & Tucker, J. (1985). Effective solvents and extraction periods employed
in phytoplankton carotenoid and chl determinations. Canadian Journal of Fisheries and Aquatic
Sciences, 42, 1127–1131.
Cartaxana, P., & Brotas, V. (2003). Effects of extraction on HPLC quantification of major pigments
from benthic microalgae. Archiv fur Hydrobiologie, 157, 339–349.
Chen, K., Rios, J. J., Pérez-Gálvez, A., & Roca, M. (2015a). Development of an accurate and highthroughput methodology for structural comprehension of chlorophylls derivatives. (I) Phytylated
derivatives. Journal of Chromatography A, 1406, 99–108.
Chen, K., Rios, J. J., Roca, M., & Pérez-Gálvez, A. (2015b). Development of an accurate and
high-throughput methodology for structural comprehension of chlorophylls derivatives. (II)
Dephytylated derivatives. Journal of Chromatography A, 1412, 90–99.
Franco, B. M., Navas, L. M., Gómez, C., Sepúlveda, C., & Acién, F. G. (2019). Monoalgal and
mixed algal cultures discrimination by using an artificial neural network. Algal Research, 38,
101419.
Freitas, S., Silva, N. G., Sousa, M. L., Ribeiro, T., Rosa, F., Leão, P. N., et al. (2019). Chlorophyll
derivatives from marine cyanobacteria with lipid-reducing activities. Marine Drugs, 17, E229.
Furuya, K., Hayashi, M., & Yabushita, Y. (1998). HPLC determination of phytoplankton pigments
using N, N-dimethyl formamide. Journal of Oceanography, 54, 199–203.
Garrido, J. L., & Roy, S. (2015). The use of HPLC for the characterization of phytoplankton
pigments. Methods in Molecular Biology, 1308, 241–252.
Garrido, J. L., & Zapata, M. (1996). Ion-pair reversed phase high-performance liquid chromatography of algal chlorophylls. Journal of Chromatography, 738, 285–289.
Garrido, J. L., & Zapata, M. (1997). Reversed-phase high-performance liquid chromatography of
mono- and divinyl chlorophyll forms using pyridine-containing mobile phases and polymeric
octadecylsilica column. Chromatographia, 44, 43–49.
Garrido, J. L., Otero, J., Maestro, M. A., & Zapata, M. (2000). The main nonpolar chlorophyll c
from Emiliana huxleyi (Prymnesiophyceae) is a chlorophyll c 2 -monogalactosyldiacylglyceride
ester: A mass spectrometry study. Journal of Phycology, 36, 497–505.
Garrido, J. L., Airs, R., Rodríguez, F., Van Heukelem, L., & Zapata, M. (2011). New HPLC separation techniques. In S. Roy, C. A. Llewellyn, E. S. Egeland, & G. Johnsen (Eds.), Phytoplankton
145
Acknowledgements This work was supported by the Comisión Interministerial de Ciencia y
Tecnología (CICYT-EU, Spanish and European Government, grant number RTI2018-095415-BI00).
References
Airs, R. L. (2018). Mass spectrometry of chlorophylls from phototrophic prokaryotes. Current
Organic Chemistry, 22, 877–884.
Airs, R., & Garrido, J. L. (2011). Liquid chromatography-mass spectrometry for pigment analysis. In S. Roy, C. A. Llewellyn, E. S. Egeland, & G. Johnsen (Eds.), Phytoplankton pigments:
Characterization, chemotaxonomy and applications in oceanography (pp. 314–342). New York:
Cambridge University Press.
Airs, R. L., Temperton, B., Sambles, B., Farnham, G., Skill, S. C., & Llewellyn, C. A. (2014).
Chlorophyll f and chlorophyll d are produced in the cyanobacterium Chlorogloeopsis fritschii
when cultured under natural light and near-infrared radiation. FEBS Letters, 588, 3770–3777.
Arnon, D. I. (1949). Copper enzymes in isolated chloroplasts: Polyphenoloxidase in Beta vulgaris.
Plant Physiology, 24, 1–15.
Barrett, J., & Jeffries, S. W. (1971). A note on the occurrence of chlorophyllase in marine. Journal
of Experimental Marine Biology and Ecology, 7, 255–262.
Bowles, N. D., Paerl, H. W., & Tucker, J. (1985). Effective solvents and extraction periods employed
in phytoplankton carotenoid and chl determinations. Canadian Journal of Fisheries and Aquatic
Sciences, 42, 1127–1131.
Cartaxana, P., & Brotas, V. (2003). Effects of extraction on HPLC quantification of major pigments
from benthic microalgae. Archiv fur Hydrobiologie, 157, 339–349.
Chen, K., Rios, J. J., Pérez-Gálvez, A., & Roca, M. (2015a). Development of an accurate and highthroughput methodology for structural comprehension of chlorophylls derivatives. (I) Phytylated
derivatives. Journal of Chromatography A, 1406, 99–108.
Chen, K., Rios, J. J., Roca, M., & Pérez-Gálvez, A. (2015b). Development of an accurate and
high-throughput methodology for structural comprehension of chlorophylls derivatives. (II)
Dephytylated derivatives. Journal of Chromatography A, 1412, 90–99.
Franco, B. M., Navas, L. M., Gómez, C., Sepúlveda, C., & Acién, F. G. (2019). Monoalgal and
mixed algal cultures discrimination by using an artificial neural network. Algal Research, 38,
101419.
Freitas, S., Silva, N. G., Sousa, M. L., Ribeiro, T., Rosa, F., Leão, P. N., et al. (2019). Chlorophyll
derivatives from marine cyanobacteria with lipid-reducing activities. Marine Drugs, 17, E229.
Furuya, K., Hayashi, M., & Yabushita, Y. (1998). HPLC determination of phytoplankton pigments
using N, N-dimethyl formamide. Journal of Oceanography, 54, 199–203.
Garrido, J. L., & Roy, S. (2015). The use of HPLC for the characterization of phytoplankton
pigments. Methods in Molecular Biology, 1308, 241–252.
Garrido, J. L., & Zapata, M. (1996). Ion-pair reversed phase high-performance liquid chromatography of algal chlorophylls. Journal of Chromatography, 738, 285–289.
Garrido, J. L., & Zapata, M. (1997). Reversed-phase high-performance liquid chromatography of
mono- and divinyl chlorophyll forms using pyridine-containing mobile phases and polymeric
octadecylsilica column. Chromatographia, 44, 43–49.
Garrido, J. L., Otero, J., Maestro, M. A., & Zapata, M. (2000). The main nonpolar chlorophyll c
from Emiliana huxleyi (Prymnesiophyceae) is a chlorophyll c 2 -monogalactosyldiacylglyceride
ester: A mass spectrometry study. Journal of Phycology, 36, 497–505.
Garrido, J. L., Airs, R., Rodríguez, F., Van Heukelem, L., & Zapata, M. (2011). New HPLC separation techniques. In S. Roy, C. A. Llewellyn, E. S. Egeland, & G. Johnsen (Eds.), Phytoplankton