144
I. Viera and M. Roca
Table 6.6 Identification of chlorophylls according to their elution order and spectral maxima
absorption
Peak Chlorophyll compound
Absorption
(nm)
Elemental
composition
Exact mass
[M+H] +
1
Chlorophyllide b
470 654
C 35 H 32 MgN 4 O 6
629.2245
2
Chlorophyll c 3
460 638
C 36 H 28 MgN 4 O 7
653.1881
3
[8-ethyl]-chlorophyll c 3
450 585
C 36 H 30 MgN 4 O 7
655.2038
4
[7-methoxycarbonyl]-8-vinyl-phlide
a
448 579
C 36 H 30 MgN 4 O 7
655.2038
5
Chlorophyllide a
432 666
C 35 H 34 MgN 4 O 5
615.2452
6
Chlorophyll c 2
450 630
C 35 H 28 MgN 4 O 5
609.1983
7
Chlorophyll c 1
446 630
C 35 H 30 N 4 O 5
611.2139
8
[8-vinyl]-phlide a
438 628
C 35 H 30 MgN 4 O 5
611.2139
9
Pheophorbide a
410 666
C 35 H 36 N 4 O 5
593.2759
10
Pyropheophorbide a
410 666
C 33 H 34 N 4 O 3
535.2704
11
MV Protochlorophyllide a
435 631
C 35 H 32 MgN 4 O 5
613.2296
12
Bacteriochlorophyll a
364 770
C 55 H 74 MgN 4 O 6
911.5532
13
DV Chlorophyll b
480 655
C 55 H 68 MgN 4 O 6
905.5062
14
13 2 -OH-chlorophyll b
466 650
C 55 H 70 MgN 4 O 7
923.5168
15
Chlorophyll b
466 651
C 55 H 70 MgN 4 O 6
907.5219
16
Chlorophyll d
447 690
C 54 H 70 MgN 4 O 6
895.5219
17
Chlorophyll f
406 705
C 55 H 70 MgN 4 O 6
907.5219
18
Chl c 2 -MGDG [18:4/14:0]
455 630
C 76 H 96 MgN 4 O 14 1313.6846
19
Chl c 2 -MGDG [14:0/14:0]
460 637
C 72 H 96 MgN 4 O 14 1265.6846
20
Chl c 1 -MGDG a
457 637
21
13 2 -OH-chlorophyll a
430 666
C 55 H 72 MgN 4 O 6
909.5375
22
DV Chlorophyll a
442 666
C 55 H 70 MgN 4 O 5
891.5269
23
Chlorophyll a
432 666
C 55 H 72 MgN 4 O 5
893.5426
24
Pheophytin b
436 650
C 55 H 72 N 4 O 6
885.5525
25
Pheophytin a
410 666
C 55 H 74 N 4 O 5
871.5732
26
Pyropheophytin a
410 666
C 53 H 22 N 4 O 3
813.5677
Bacteriochlorophyll b
368 796
C 55 H 72 MgN 4 O 6
909.5375
Bacteriochlorophyll c b
432 666
Bacteriochlorophyll d b
427 665
Bacteriochlorophyll e b
469 654
Bacteriochlorophyll g
365 766
C 50 H 58 MgN 4 O 5
819.4330
a At the moment no fatty acid has been identified. b Bacteriochlorophyll c, d, and e exhibit a diverse
elemental composition and polarity in function of the substituents, but usually they are less polar
than chlorophyll a
I. Viera and M. Roca
Table 6.6 Identification of chlorophylls according to their elution order and spectral maxima
absorption
Peak Chlorophyll compound
Absorption
(nm)
Elemental
composition
Exact mass
[M+H] +
1
Chlorophyllide b
470 654
C 35 H 32 MgN 4 O 6
629.2245
2
Chlorophyll c 3
460 638
C 36 H 28 MgN 4 O 7
653.1881
3
[8-ethyl]-chlorophyll c 3
450 585
C 36 H 30 MgN 4 O 7
655.2038
4
[7-methoxycarbonyl]-8-vinyl-phlide
a
448 579
C 36 H 30 MgN 4 O 7
655.2038
5
Chlorophyllide a
432 666
C 35 H 34 MgN 4 O 5
615.2452
6
Chlorophyll c 2
450 630
C 35 H 28 MgN 4 O 5
609.1983
7
Chlorophyll c 1
446 630
C 35 H 30 N 4 O 5
611.2139
8
[8-vinyl]-phlide a
438 628
C 35 H 30 MgN 4 O 5
611.2139
9
Pheophorbide a
410 666
C 35 H 36 N 4 O 5
593.2759
10
Pyropheophorbide a
410 666
C 33 H 34 N 4 O 3
535.2704
11
MV Protochlorophyllide a
435 631
C 35 H 32 MgN 4 O 5
613.2296
12
Bacteriochlorophyll a
364 770
C 55 H 74 MgN 4 O 6
911.5532
13
DV Chlorophyll b
480 655
C 55 H 68 MgN 4 O 6
905.5062
14
13 2 -OH-chlorophyll b
466 650
C 55 H 70 MgN 4 O 7
923.5168
15
Chlorophyll b
466 651
C 55 H 70 MgN 4 O 6
907.5219
16
Chlorophyll d
447 690
C 54 H 70 MgN 4 O 6
895.5219
17
Chlorophyll f
406 705
C 55 H 70 MgN 4 O 6
907.5219
18
Chl c 2 -MGDG [18:4/14:0]
455 630
C 76 H 96 MgN 4 O 14 1313.6846
19
Chl c 2 -MGDG [14:0/14:0]
460 637
C 72 H 96 MgN 4 O 14 1265.6846
20
Chl c 1 -MGDG a
457 637
21
13 2 -OH-chlorophyll a
430 666
C 55 H 72 MgN 4 O 6
909.5375
22
DV Chlorophyll a
442 666
C 55 H 70 MgN 4 O 5
891.5269
23
Chlorophyll a
432 666
C 55 H 72 MgN 4 O 5
893.5426
24
Pheophytin b
436 650
C 55 H 72 N 4 O 6
885.5525
25
Pheophytin a
410 666
C 55 H 74 N 4 O 5
871.5732
26
Pyropheophytin a
410 666
C 53 H 22 N 4 O 3
813.5677
Bacteriochlorophyll b
368 796
C 55 H 72 MgN 4 O 6
909.5375
Bacteriochlorophyll c b
432 666
Bacteriochlorophyll d b
427 665
Bacteriochlorophyll e b
469 654
Bacteriochlorophyll g
365 766
C 50 H 58 MgN 4 O 5
819.4330
a At the moment no fatty acid has been identified. b Bacteriochlorophyll c, d, and e exhibit a diverse
elemental composition and polarity in function of the substituents, but usually they are less polar
than chlorophyll a