6 Analytical Protocols in Chlorophyll Analysis
137
Table 6.2 Green protocols
for chlorophylls extraction
from phytoplankton
Organism
Extraction method References
Cylindrotheca
closterium
MAE
Pasquet et al.
(2011)
Dunaliella tertiolecta MAE
Pasquet et al.
(2011)
Cylindrotheca
closterium
UAE
Pasquet et al.
(2011)
Dunaliella salina
UAE
Macías-Sánchez
et al. (2009)
Dunaliella tertiolecta UAE
Pasquet et al.
(2011)
Nannochloropsis
gaditana
UAE
Macías-Sánchez
et al. (2005)
Macías-Sánchez
et al. (2008)
Synechococcus sp.
UAE
Macías-Sánchez
et al. (2007)
Chlorococcum
littorale
SFE
Ota et al. (2009)
Dunaliella salina
SFE
Jaime et al.
(2007)
Macías-Sánchez
et al. (2009)
Macías-Sánchez
et al. (2008)
Nannochloropsis sp. SFE
Nobre et al. 2013
Nannochloropsis
gaditana
SFE
Macías-Sánchez
et al. (2005)
Macías-Sánchez
et al. (2008)
Synechococcus sp.
SFE
Montero et al.
(2005)
Macías-Sánchez
et al. (2007)
Macías-Sánchez
et al. (2008)
Chlorella vulgaris
PLE
Santoyo et al.
(2010)
aim pursued. Fundamentally, the analysis of chlorophylls from phytoplankton faces
three problems: separation of monovinylic chlorophylls (chlorophyll a, chlorophyll b,
chlorophyll c 1 , and MV-chlorophyll c 3 ) from their counterparts divinyl chlorophylls
(DV-chl a, DV-chl b, chl c 2 , and chl c 3 ), separation of highly polar chlorophylls
(chlorophyllide, pheophorbide, and different chlorophyll c), and separation between
bacteriochlorophylls (mainly, bacteriochlorophyll c, d, and e).