Table 1
List of available zebrafish transgenic lines to monitor NC development
Line name
Allele
Description
Regulatory element References
FoxD3 promotor
foxd3:GFP
zf15Tg
Cytoplasmic GFP, not strongly expressed in NC before 24hpf.
14 zFoxD3
[15]
foxd3-citrine
ct110aGt
Cytoplasmic fluorescent protein under the control of endogenous
promotor. Gene/trap screen. Earliest reporter of NC, expressed
from neurula stages. Downregulated by 24hpf and expressed
in muscle cells from
%20hpf. Homozygous embryos are null mutants.
Heterozygous do not show phenotype and can be used as reporter.
Endogenous
[16]
foxd3-mCherry
ct110aRGt
Crestin promoto
Crestin:EGFP
cz3337Tg
Cytoplasmic EGFP.
4.5 Crestin
[17]
Crestin:Gal4-VP16,14x
UAS-E1b:EGFP
t32231Tg
Cytoplasmic GFP driven through Gal4 activation. Not strongly
expressed in NC before 24hpf. Expressed in dorsal EVL
1 Crestin
Sox10 promotor
Cytoplasmic
7.2sox10:EGFP
ir937Tg
Cytoplasmic EGFP
7.2 Sox10
[18]
4.9Sox10:GFP
ba2Tg
Cytoplasmic EGFP
4.9Sox10
4.725Sox10:GFP
ba3Tg
Cytoplasmic EGFP
4.7 Sox10
4.725Sox10:GFP
ba4Tg
Cytoplasmic EGFP
4.7 Sox11
[19]
1252Sox10:GFP
ba5Tg
Cytoplasmic EGFP
1252 Sox10
sox10:DsRed
el10Tg
Cytoplasmic RFP
4.9 Sox10
[20]
sox10:TagRFP
co26Tg
Cytoplasmic RFP
7.2 Sox10
[21]
Membrane
sox10:EGFP
CAAX
el375Tg
Membrane EGFP
4.9 Sox10
[22]
sox10:mCherryCAAX
el361Tg
Membrane RFP
4.9 Sox10
4.9sox10:LY-tomato
ir1040Tg
Membrane RFP
4.9 Sox10
[23]
4.9sox10:LY-GFP
ir866Tg
Membrane GFP
4.9 Sox10
sox10:EGFP-Hsa.HRAS
sl3Tg
Membrane EGFP
7.2 Sox10
[24]
82
Zain Alhashem et al.