32. Mueller F, Morisaki T, Mazza D, McNally JG
(2012) Minimizing the impact of photoswitching of fluorescent proteins on FRAP analysis.
Biophys J 102:1656–1665
33. Jian X, Cavenagh M, Gruschus JM, Randazzo
PA, Kahn RA (2010) Modifications to the
C-terminus of Arf1 alter cell functions and
protein interactions. Traffic 11:732–742
34. Hoffmann A, Dannhauser PN, Groos S,
Hinrichsen L, Curth U, Ungewickell EJ
(2010) A comparison of GFP-tagged clathrin
light chains with fluorochromated light chains
in vivo and in vitro. Traffic 11:1129–1140
35. Blumenthal D, Goldstien L, Edidin M, Gheber
LA (2015) Universal approach to FRAP analysis of arbitrary bleaching patterns. Sci Rep
5:11655
36. Huang J, Huang L, Chen Y-J, Austin E, Devor
CE, Roegiers F, Hong Y (2011) Differential
regulation of adherens junction dynamics during apical–basal polarization. J Cell Sci
124:4001–4013
37. Axelrod D (1977) Cell surface heating during
fluorescence photobleaching recovery experiments. Biophys J 18:129–131
38. Lalo U, Allsopp RC, Mahaut-Smith MP, Evans
RJ (2010) P2X1 receptor mobility and trafficking; regulation by receptor insertion and activation. J Neurochem 113:1177–1187
39. Weiss M (2004) Challenges and artifacts in
quantitative photobleaching experiments.
Traffic 5:662–671
40. Wu J, Shekhar N, Lele PP, Lele TP (2012)
FRAP analysis: accounting for bleaching during image capture. PLoS One 7:e42854
41. Iyer KV, Piscitello-Go ´ mez R, Paijmans J,
Ju ¨licher F, Eaton S (2019) Epithelial viscoelasticity is regulated by mechanosensitive
E-cadherin turnover. Curr Biol 29:578–591.e5
42. Parks PC (1992) A. M. Lyapunov’s stability
theory—100 years on. IMA J Math Control
Inf 9:275–303
43. Lyapunov AM (1992) The general problem of
the stability of motion. Int J Control
55:531–534
44. Bulinski JC, Odde DJ, Howell BJ, Salmon TD,
Waterman-Storer CM (2001) Rapid dynamics
of the microtubule binding of ensconsin
in vivo. J Cell Sci 114:3885–3897
FRAP to Study the Dynamics of Proteins in vivo
159
(2012) Minimizing the impact of photoswitching of fluorescent proteins on FRAP analysis.
Biophys J 102:1656–1665
33. Jian X, Cavenagh M, Gruschus JM, Randazzo
PA, Kahn RA (2010) Modifications to the
C-terminus of Arf1 alter cell functions and
protein interactions. Traffic 11:732–742
34. Hoffmann A, Dannhauser PN, Groos S,
Hinrichsen L, Curth U, Ungewickell EJ
(2010) A comparison of GFP-tagged clathrin
light chains with fluorochromated light chains
in vivo and in vitro. Traffic 11:1129–1140
35. Blumenthal D, Goldstien L, Edidin M, Gheber
LA (2015) Universal approach to FRAP analysis of arbitrary bleaching patterns. Sci Rep
5:11655
36. Huang J, Huang L, Chen Y-J, Austin E, Devor
CE, Roegiers F, Hong Y (2011) Differential
regulation of adherens junction dynamics during apical–basal polarization. J Cell Sci
124:4001–4013
37. Axelrod D (1977) Cell surface heating during
fluorescence photobleaching recovery experiments. Biophys J 18:129–131
38. Lalo U, Allsopp RC, Mahaut-Smith MP, Evans
RJ (2010) P2X1 receptor mobility and trafficking; regulation by receptor insertion and activation. J Neurochem 113:1177–1187
39. Weiss M (2004) Challenges and artifacts in
quantitative photobleaching experiments.
Traffic 5:662–671
40. Wu J, Shekhar N, Lele PP, Lele TP (2012)
FRAP analysis: accounting for bleaching during image capture. PLoS One 7:e42854
41. Iyer KV, Piscitello-Go ´ mez R, Paijmans J,
Ju ¨licher F, Eaton S (2019) Epithelial viscoelasticity is regulated by mechanosensitive
E-cadherin turnover. Curr Biol 29:578–591.e5
42. Parks PC (1992) A. M. Lyapunov’s stability
theory—100 years on. IMA J Math Control
Inf 9:275–303
43. Lyapunov AM (1992) The general problem of
the stability of motion. Int J Control
55:531–534
44. Bulinski JC, Odde DJ, Howell BJ, Salmon TD,
Waterman-Storer CM (2001) Rapid dynamics
of the microtubule binding of ensconsin
in vivo. J Cell Sci 114:3885–3897
FRAP to Study the Dynamics of Proteins in vivo
159