planes within a Z-stack typically allow reconstructing cell shape
in 3D. Embryos are ideally imaged for around 8–12 h between
E7 and E7.75. Embryos should then be cultured for an additional 6–12 h after imaging to check for fitness.
References
1. Arnold SJ, Robertson EJ (2009) Making a
commitment: cell lineage allocation and axis
patterning in the early mouse embryo. Nat
Rev Mol Cell Biol 10:91–103
2. Ramkumar N, Anderson KV (2011) SnapShot:
mouse primitive streak. Cell 146:488–488.e2
3. Nahaboo W, Migeotte I (2018) Cleavage and
gastrulation in the mouse embryo. eLS:1–6
4. Ramkumar N, Omelchenko T, Silva-Gagliardi
NF, McGlade CJ, Wijnholds J, Anderson KV
(2016) Crumbs2 promotes cell ingression during the epithelial-to-mesenchymal transition at
gastrulation. Nat Cell Biol 18(12):1281–1291
5. Williams M, Burdsal C, Periasamy A,
Lewandoski M, Sutherland A (2012) The
mouse primitive streak forms in situ by initiation of epithelial to mesenchymal transition
without migration of a cell population. Dev
Dyn 241:270–283
6. Ferrer-Vaquer A, Viotti M, Hadjantonakis A-K
(2010) Transitions between epithelial and mesenchymal states and the morphogenesis of the
early mouse embryo. Cell Adhes Migr
4:447–457
7. Srinivas S (2010) Imaging cell movements in
egg-cylinder stage mouse embryos. Cold
Spring Harb Protoc 5:1386–1394
8. Nowotschin S, Garg V, Piliszek A, Hadjantonakis A-K (2019) Ex utero culture and imaging
of mouse embryos BT. In: Vertebrate embryogenesis: embryological, cellular, and genetic
methods. Springer, New York, pp 163–182
9. McDole K, Guignard L, Amat F, Berger A,
Malandain G, Royer LA et al (2018) In Toto
imaging and reconstruction of postimplantation mouse development at the
single-cell level. Cell 175:1–18
10. Gosse C, Zhao X, Migeotte I, Sua ´rezBoomgaard D, Hue I, Degrelle S et al (2017)
The use of electroporation in developmental
biology BT. In: Handbook of electroporation.
Springer International Publishing, New York,
pp 1375–1409
11. Muzumdar MD, Tasic B, Miyamichi K, Li L,
Luo L (2007) A global double-fluorescent Cre
reporter mouse. Genesis 45:593–605
12. Arnold K, Sarkar A, Yram MA, Polo JM,
Sengupta S, Seandel M et al (2013) Regeneration and survival of mice. Cell Stem Cell
9:317–329
13. Kiyonari H, Kaneko M, Abe T, Shioi G,
Aizawa S, Furuta Y et al (2019) Dynamic
organelle localization and cytoskeletal reorganization during preimplantation mouse
embryo development revealed by live imaging
of genetically encoded fluorescent fusion proteins. Genesis 57:1–9
14. Saykali B, Mathiah N, Nahaboo W, Racu M-L,
Hammou L, Defrance M et al (2019) Distinct
mesoderm migration phenotypes in extraembryonic and embryonic regions of the early
mouse embryo. Elife 8:e42434
15. Downs KM, Davies T (1993) Staging of gastrulating mouse embryos by morphological
landmarks in the dissecting microscope. Development 118:1255–1266
144
Wallis Nahaboo et al.
in 3D. Embryos are ideally imaged for around 8–12 h between
E7 and E7.75. Embryos should then be cultured for an additional 6–12 h after imaging to check for fitness.
References
1. Arnold SJ, Robertson EJ (2009) Making a
commitment: cell lineage allocation and axis
patterning in the early mouse embryo. Nat
Rev Mol Cell Biol 10:91–103
2. Ramkumar N, Anderson KV (2011) SnapShot:
mouse primitive streak. Cell 146:488–488.e2
3. Nahaboo W, Migeotte I (2018) Cleavage and
gastrulation in the mouse embryo. eLS:1–6
4. Ramkumar N, Omelchenko T, Silva-Gagliardi
NF, McGlade CJ, Wijnholds J, Anderson KV
(2016) Crumbs2 promotes cell ingression during the epithelial-to-mesenchymal transition at
gastrulation. Nat Cell Biol 18(12):1281–1291
5. Williams M, Burdsal C, Periasamy A,
Lewandoski M, Sutherland A (2012) The
mouse primitive streak forms in situ by initiation of epithelial to mesenchymal transition
without migration of a cell population. Dev
Dyn 241:270–283
6. Ferrer-Vaquer A, Viotti M, Hadjantonakis A-K
(2010) Transitions between epithelial and mesenchymal states and the morphogenesis of the
early mouse embryo. Cell Adhes Migr
4:447–457
7. Srinivas S (2010) Imaging cell movements in
egg-cylinder stage mouse embryos. Cold
Spring Harb Protoc 5:1386–1394
8. Nowotschin S, Garg V, Piliszek A, Hadjantonakis A-K (2019) Ex utero culture and imaging
of mouse embryos BT. In: Vertebrate embryogenesis: embryological, cellular, and genetic
methods. Springer, New York, pp 163–182
9. McDole K, Guignard L, Amat F, Berger A,
Malandain G, Royer LA et al (2018) In Toto
imaging and reconstruction of postimplantation mouse development at the
single-cell level. Cell 175:1–18
10. Gosse C, Zhao X, Migeotte I, Sua ´rezBoomgaard D, Hue I, Degrelle S et al (2017)
The use of electroporation in developmental
biology BT. In: Handbook of electroporation.
Springer International Publishing, New York,
pp 1375–1409
11. Muzumdar MD, Tasic B, Miyamichi K, Li L,
Luo L (2007) A global double-fluorescent Cre
reporter mouse. Genesis 45:593–605
12. Arnold K, Sarkar A, Yram MA, Polo JM,
Sengupta S, Seandel M et al (2013) Regeneration and survival of mice. Cell Stem Cell
9:317–329
13. Kiyonari H, Kaneko M, Abe T, Shioi G,
Aizawa S, Furuta Y et al (2019) Dynamic
organelle localization and cytoskeletal reorganization during preimplantation mouse
embryo development revealed by live imaging
of genetically encoded fluorescent fusion proteins. Genesis 57:1–9
14. Saykali B, Mathiah N, Nahaboo W, Racu M-L,
Hammou L, Defrance M et al (2019) Distinct
mesoderm migration phenotypes in extraembryonic and embryonic regions of the early
mouse embryo. Elife 8:e42434
15. Downs KM, Davies T (1993) Staging of gastrulating mouse embryos by morphological
landmarks in the dissecting microscope. Development 118:1255–1266
144
Wallis Nahaboo et al.