6 Summary
Table 1 provides a summary of total peptides and proteins identified after proteome extraction of 4 single colonies.
References
1. Sa ´nchez-Romero MA, Casadesu ´ s J (2014) Contribution of phenotypic heterogeneity to adaptive antibiotic resistance. Proc Natl Acad Sci U S
A 111(1):355–360. https://doi.org/10.1073/
pnas.1316084111
2. Stockholm D, Benchaouir R, Picot J et al (2007)
The origin of phenotypic heterogeneity in a
clonal cell population in vitro. PLoS One 2(4):
e394. https://doi.org/10.1371/journal.pone.
0000394
3. Feist P, Hummon AB (2015) Proteomic challenges: sample preparation techniques for
microgram-quantity protein analysis from
biological samples. Int J Mol Sci 16
(2):3537–3563
4. Zhou J, Zhang L, Chuan H et al (2019) Mass
spectrometry to study the bacterial proteome
from a single Colony. Methods Mol Biol
1968:113–121
5. Kalule JB, Fortuin S, Calder B et al (2018)
Proteomic comparison of three clinical diarrhoeagenic drug-resistant Escherichia coli isolates grown on CHROMagar™STEC media. J
Proteome 180:25–35. https://doi.org/10.
1016/j.jprot.2017.09.003
6. Rappsilber J, Ishihama Y, Mann M (2003) Stop
and go extraction tips for matrix-assisted laser
desorption/ionization, nanoelectrospray, and
LC/MS sample pretreatment in proteomics.
Anal Chem 75(3):663–670. https://doi.org/
10.1021/ac026117i
7. Tyanova S, Temu T, Sinitcyn P et al (2016) The
Perseus computational platform for comprehensive analysis of (prote)omics data. Nat Methods
13(9):731–740
8. Tyanova S, Cox J (2018) Perseus: a bioinformatics platform for integrative analysis of proteomics data in cancer research. Methods Mol Biol
1711:133–148
Mass Spectrometry-Based Analysis of Mycobacterial Single-Colony Proteome
189
Table 1 provides a summary of total peptides and proteins identified after proteome extraction of 4 single colonies.
References
1. Sa ´nchez-Romero MA, Casadesu ´ s J (2014) Contribution of phenotypic heterogeneity to adaptive antibiotic resistance. Proc Natl Acad Sci U S
A 111(1):355–360. https://doi.org/10.1073/
pnas.1316084111
2. Stockholm D, Benchaouir R, Picot J et al (2007)
The origin of phenotypic heterogeneity in a
clonal cell population in vitro. PLoS One 2(4):
e394. https://doi.org/10.1371/journal.pone.
0000394
3. Feist P, Hummon AB (2015) Proteomic challenges: sample preparation techniques for
microgram-quantity protein analysis from
biological samples. Int J Mol Sci 16
(2):3537–3563
4. Zhou J, Zhang L, Chuan H et al (2019) Mass
spectrometry to study the bacterial proteome
from a single Colony. Methods Mol Biol
1968:113–121
5. Kalule JB, Fortuin S, Calder B et al (2018)
Proteomic comparison of three clinical diarrhoeagenic drug-resistant Escherichia coli isolates grown on CHROMagar™STEC media. J
Proteome 180:25–35. https://doi.org/10.
1016/j.jprot.2017.09.003
6. Rappsilber J, Ishihama Y, Mann M (2003) Stop
and go extraction tips for matrix-assisted laser
desorption/ionization, nanoelectrospray, and
LC/MS sample pretreatment in proteomics.
Anal Chem 75(3):663–670. https://doi.org/
10.1021/ac026117i
7. Tyanova S, Temu T, Sinitcyn P et al (2016) The
Perseus computational platform for comprehensive analysis of (prote)omics data. Nat Methods
13(9):731–740
8. Tyanova S, Cox J (2018) Perseus: a bioinformatics platform for integrative analysis of proteomics data in cancer research. Methods Mol Biol
1711:133–148
Mass Spectrometry-Based Analysis of Mycobacterial Single-Colony Proteome
189