Total Carbohydrate Content Determination of Microalgal
Biomass by Acid Hydrolysis Followed by Spectrophotometry
or Liquid Chromatography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 191
S. Van Wychen and L. M. L. Laurens
Total Fatty Acid Content Determination of Whole Microalgal
Biomass Using In Situ Transesterification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203
S. Van Wychen and L. M. L. Laurens
Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Analysis
of Molecular Lipids in Algae Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 215
Heli Nygren, Tuulikki Sepp€ anen-Laakso, and Heiko Rischer
UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization
of Bound and Free Fatty Acids and Sterols for GC-MS Methods . . . . . . . . . . . . . . . . . . 223
Tuulikki Sepp€ anen-Laakso, Heli Nygren, and Heiko Rischer
Total Protein Content Determination of Microalgal Biomass by Elemental
Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor . . . . . . . . 233
L. M. L. Laurens, J. L. Olstad, and D. W. Templeton
Correction to: Determining Inorganic and Organic Nitrogen. . . . . . . . . . . . . . . . . . . . . 243
Jaana Koistinen, Mervi Sjo ¨blom, and Kristian Spilling
Correction to: Determining Inorganic and Organic Phosphorus . . . . . . . . . . . . . . . . . . 245
Jaana Koistinen, Mervi Sjo ¨blom, and Kristian Spilling
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 247
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Contents
Biomass by Acid Hydrolysis Followed by Spectrophotometry
or Liquid Chromatography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 191
S. Van Wychen and L. M. L. Laurens
Total Fatty Acid Content Determination of Whole Microalgal
Biomass Using In Situ Transesterification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203
S. Van Wychen and L. M. L. Laurens
Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Analysis
of Molecular Lipids in Algae Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 215
Heli Nygren, Tuulikki Sepp€ anen-Laakso, and Heiko Rischer
UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization
of Bound and Free Fatty Acids and Sterols for GC-MS Methods . . . . . . . . . . . . . . . . . . 223
Tuulikki Sepp€ anen-Laakso, Heli Nygren, and Heiko Rischer
Total Protein Content Determination of Microalgal Biomass by Elemental
Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor . . . . . . . . 233
L. M. L. Laurens, J. L. Olstad, and D. W. Templeton
Correction to: Determining Inorganic and Organic Nitrogen. . . . . . . . . . . . . . . . . . . . . 243
Jaana Koistinen, Mervi Sjo ¨blom, and Kristian Spilling
Correction to: Determining Inorganic and Organic Phosphorus . . . . . . . . . . . . . . . . . . 245
Jaana Koistinen, Mervi Sjo ¨blom, and Kristian Spilling
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 247
viii
Contents