Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
v
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
ix
Large Scale Cultivation of Microalgae: Open and Closed Systems . . . . . . . . . . . . . . . . .
1
Jacqueline Jerney and Kristian Spilling
Design and Analysis of Offshore Macroalgae Biorefineries . . . . . . . . . . . . . . . . . . . . . . . .
9
Alexander Golberg, Alexander Liberzon, Edward Vitkin,
and Zohar Yakhini
Basic Methods for Isolating and Culturing Microalgae . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Kristian Spilling
Measurement of Fluorescence for Monitoring Algal Growth and Health . . . . . . . . . . . 41
Kristian Spilling and Jukka Sepp€ al€ a
Microalgae Lipid Staining with Fluorescent BODIPY Dye. . . . . . . . . . . . . . . . . . . . . . . . 47
Judita Koreiviene ˙
Monitoring Microalgal Neutral Lipid Accumulation with Nile Red . . . . . . . . . . . . . . . . 55
Katariina Natunen
Determining Inorganic and Organic Carbon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Jaana Koistinen, Mervi Sjo ¨blom, and Kristian Spilling
Determining Inorganic and Organic Nitrogen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Jaana Koistinen, Mervi Sjo ¨blom, and Kristian Spilling
Total Nitrogen Determination by a Spectrophotometric Method . . . . . . . . . . . . . . . . . 81
Jaana Koistinen, Mervi Sjo ¨blom, and Kristian Spilling
Determining Inorganic and Organic Phosphorus. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Jaana Koistinen, Mervi Sjo ¨blom, and Kristian Spilling
Determining Dissolved and Biogenic Silica . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
Jaana Koistinen, Mervi Sjo ¨blom, and Kristian Spilling
Life Cycle Assessment of Seaweed Cultivation Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
Michele Seghetta and Pietro Goglio
Life-Cycle Assessment (LCA) Analysis of Algal Fuels . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
Homa Hosseinzadeh-Bandbafha, Meisam Tabatabaei,
Mortaza Aghbashlo, Alawi Sulaiman, and Abbas Ghassemi
Metabolic Engineering of Microalgae for Biofuel Production . . . . . . . . . . . . . . . . . . . . . 153
Mohammad Pooya Naghshbandi, Meisam Tabatabaei,
Mortaza Aghbashlo, Muhammad Nauman Aftab, and Irfana Iqbal
Analytical Grade Purification of Phycocyanin from Cyanobacteria . . . . . . . . . . . . . . . . . 173
Mahammed Ilyas Khazi, Zeliha Demirel, Fakhra Liaqat,
and Meltem Conk Dalay
Complete Acid-Based Hydrolysis Assay for Carbohydrate
Quantification in Seaweed: A Species-Specific Optimized Approach . . . . . . . . . . . . . . . 181
Emily T. Kostas, Stuart J. Wilkinson, Daniel A. White, and David J. Cook
vii
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
v
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
ix
Large Scale Cultivation of Microalgae: Open and Closed Systems . . . . . . . . . . . . . . . . .
1
Jacqueline Jerney and Kristian Spilling
Design and Analysis of Offshore Macroalgae Biorefineries . . . . . . . . . . . . . . . . . . . . . . . .
9
Alexander Golberg, Alexander Liberzon, Edward Vitkin,
and Zohar Yakhini
Basic Methods for Isolating and Culturing Microalgae . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Kristian Spilling
Measurement of Fluorescence for Monitoring Algal Growth and Health . . . . . . . . . . . 41
Kristian Spilling and Jukka Sepp€ al€ a
Microalgae Lipid Staining with Fluorescent BODIPY Dye. . . . . . . . . . . . . . . . . . . . . . . . 47
Judita Koreiviene ˙
Monitoring Microalgal Neutral Lipid Accumulation with Nile Red . . . . . . . . . . . . . . . . 55
Katariina Natunen
Determining Inorganic and Organic Carbon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Jaana Koistinen, Mervi Sjo ¨blom, and Kristian Spilling
Determining Inorganic and Organic Nitrogen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Jaana Koistinen, Mervi Sjo ¨blom, and Kristian Spilling
Total Nitrogen Determination by a Spectrophotometric Method . . . . . . . . . . . . . . . . . 81
Jaana Koistinen, Mervi Sjo ¨blom, and Kristian Spilling
Determining Inorganic and Organic Phosphorus. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Jaana Koistinen, Mervi Sjo ¨blom, and Kristian Spilling
Determining Dissolved and Biogenic Silica . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
Jaana Koistinen, Mervi Sjo ¨blom, and Kristian Spilling
Life Cycle Assessment of Seaweed Cultivation Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
Michele Seghetta and Pietro Goglio
Life-Cycle Assessment (LCA) Analysis of Algal Fuels . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
Homa Hosseinzadeh-Bandbafha, Meisam Tabatabaei,
Mortaza Aghbashlo, Alawi Sulaiman, and Abbas Ghassemi
Metabolic Engineering of Microalgae for Biofuel Production . . . . . . . . . . . . . . . . . . . . . 153
Mohammad Pooya Naghshbandi, Meisam Tabatabaei,
Mortaza Aghbashlo, Muhammad Nauman Aftab, and Irfana Iqbal
Analytical Grade Purification of Phycocyanin from Cyanobacteria . . . . . . . . . . . . . . . . . 173
Mahammed Ilyas Khazi, Zeliha Demirel, Fakhra Liaqat,
and Meltem Conk Dalay
Complete Acid-Based Hydrolysis Assay for Carbohydrate
Quantification in Seaweed: A Species-Specific Optimized Approach . . . . . . . . . . . . . . . 181
Emily T. Kostas, Stuart J. Wilkinson, Daniel A. White, and David J. Cook
vii