3.1.2 Microarray
Genome-wide analysis of miRNA expression can be performed by microarray.
Microarray technology is a robust high-throughput method used to analyse simultaneously the expression of thousands of small non-coding RNAs, including miRNAs.
Currently, a wide range of commercial platforms based on different technologies are
available for global miRNA expression profiling, such as oligonucleotide microarray, LNA arrays (Exiqon), bead-based technology (Illumina) and microfluidic systems (Agilent, LC Biosciences). All these platforms have designed probes specific
for mature miRNA sequences. The main differences are in the hybridization phase,
Fig. 7 Schematic representation of miRNA quantification by Ligo-miR
548
C. Dell’Aversana et al.
Genome-wide analysis of miRNA expression can be performed by microarray.
Microarray technology is a robust high-throughput method used to analyse simultaneously the expression of thousands of small non-coding RNAs, including miRNAs.
Currently, a wide range of commercial platforms based on different technologies are
available for global miRNA expression profiling, such as oligonucleotide microarray, LNA arrays (Exiqon), bead-based technology (Illumina) and microfluidic systems (Agilent, LC Biosciences). All these platforms have designed probes specific
for mature miRNA sequences. The main differences are in the hybridization phase,
Fig. 7 Schematic representation of miRNA quantification by Ligo-miR
548
C. Dell’Aversana et al.