Table 17–13
Comparison of Thermostability and Other Properties of Enzymes from Mesophilic and Thermophilic Bacteria
Half-Cystine
Molecular
Metal Required
Species
Enzyme
(%)
Heat Stability
∗
(mole/mole of Protein)
Weight
for Stability
B. subtilis
Subtilisin BPN’
45
(50
◦
C, 30 minutes)
0
28,000
Yes
B. subtilis
Neutral protease
50
(60
◦
C, 15 minutes)
0
44,700
Yes
P. aeruginosa
Alkaline protease
80
(60
◦
C, 10 minutes)
0
48,400
Yes
P. aeruginosa
Elastase
86
(70
◦
C, 10 minutes)
4.6
39,500
Yes
10
(75
◦
C, 10 minutes)
Group A streptococci
Streptococcal protease
0
(70
◦
C, 30 minutes)
1
32,000
C. histolyticum
Collagenase
1.5
(50
◦
C, 20 minutes)
0
90,000
S. griseus
Pronase
60
(60
◦
C, 10 minutes)
Yes
B. thermoproteolyticus
Thermolysin
95
(60
◦
C, 120 minutes)
0
42,700
Yes
50
(80
◦
C, 60 minutes)
B. subtilis
α-Amylase
55
(65
◦
C, 20 minutes)
0
50,000
Yes
G. stearothermophilus
α-Amylase
100
(70
◦
C, 24 hours)
4
15,500
∗
Activity remaining after heat treatment shown in parentheses.
Source: Matsubara, 34
copyright c
1967 by The American Association for the Advancement of Science.
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