234 Modern Food Microbiology
Figure 10–3 Quantification of Campylobacter viability. Comparison of plate counts (5% sheep blood agar). ():
DVC assaying protein synthesis in the absence of DNA replication (᭡); and AODC (•) as indices of viability for
stream-water stationary microcosms. Source: Rollins and Colwell,
109 Copyright c
1986 American Society for
Microbiology.
became VBNC after starvation had fluorescence intensities that were 90–120% of nonstarved cells.
71
Since dead cells did not fluoresce, these findings indicate that VBNC cells retain their viability. When
Vibrio harveyi and V. fischeri were induced into the VBNC state by nutrient limitation, both lost the
capacity of luminesce but luminescence was restored when nutrient limitation was relieved by the
addition of nutrients (103).
Figure 10–3 Quantification of Campylobacter viability. Comparison of plate counts (5% sheep blood agar). ():
DVC assaying protein synthesis in the absence of DNA replication (᭡); and AODC (•) as indices of viability for
stream-water stationary microcosms. Source: Rollins and Colwell,
109 Copyright c
1986 American Society for
Microbiology.
became VBNC after starvation had fluorescence intensities that were 90–120% of nonstarved cells.
71
Since dead cells did not fluoresce, these findings indicate that VBNC cells retain their viability. When
Vibrio harveyi and V. fischeri were induced into the VBNC state by nutrient limitation, both lost the
capacity of luminesce but luminescence was restored when nutrient limitation was relieved by the
addition of nutrients (103).