Chapter 6
Estimation Using the Microcosm N-System
Kunihiko Kakazu, Kakeru Ruike, Ken-ichi Shibata,
and Kazuhito Murakami
Abstract In this chapter, the methods for measuring biomass and calculating the
amount of production and consumption and an endpoint for a standard ecosystem
impact assessment are described.
6.1 Biomass Measurement in the Microcosm N-System
Biomass should be measured by the method described herein 14 days (336 h) after
the addition of the test materials, and the quantity of the test materials should be
measured at the same time.
The constituent species of this microcosm are as follows:
– Aeolosoma hemprichi (oligochaete predator/detritus feeder)
– Philodina erythrophthalma (rotifer predator/bacteria feeder)
– Lecane sp. (rotifer predator/bacterivore)
– Cyclidium glaucoma (ciliate predator/bacterivore)
– Chlorella sp. (chlorophycean producer)
– Scenedesmus quadricauda (chlorophycean producer)
– Tolypothrix sp. (cyanophycean producer)
K. Kakazu
Foundation for Advancement of International Science, Tsukuba, Ibaraki, Japan
e-mail: kakazu@fais.or.jp
K. Ruike
University of Tsukuba/Foundation for Advancement of International Science, Tsukuba, Ibaraki,
Japan
e-mail: ruike@fais.or.jp
K. Shibata
Yokohama National University, Yokohama, Kanagawa, Japan
e-mail: shibata-kenichi-ym@ynu.jp
K. Murakami (*)
Chiba Institute of Technology, Narashino, Chiba, Japan
e-mail: kazuhito.murakami@p.chibakoudai.jp
© Springer Nature Singapore Pte Ltd. 2020
Y. Inamori (ed.), Microcosm Manual for Environmental Impact Risk Assessment,
https://doi.org/10.1007/978-981-13-6798-4_6
45
Estimation Using the Microcosm N-System
Kunihiko Kakazu, Kakeru Ruike, Ken-ichi Shibata,
and Kazuhito Murakami
Abstract In this chapter, the methods for measuring biomass and calculating the
amount of production and consumption and an endpoint for a standard ecosystem
impact assessment are described.
6.1 Biomass Measurement in the Microcosm N-System
Biomass should be measured by the method described herein 14 days (336 h) after
the addition of the test materials, and the quantity of the test materials should be
measured at the same time.
The constituent species of this microcosm are as follows:
– Aeolosoma hemprichi (oligochaete predator/detritus feeder)
– Philodina erythrophthalma (rotifer predator/bacteria feeder)
– Lecane sp. (rotifer predator/bacterivore)
– Cyclidium glaucoma (ciliate predator/bacterivore)
– Chlorella sp. (chlorophycean producer)
– Scenedesmus quadricauda (chlorophycean producer)
– Tolypothrix sp. (cyanophycean producer)
K. Kakazu
Foundation for Advancement of International Science, Tsukuba, Ibaraki, Japan
e-mail: kakazu@fais.or.jp
K. Ruike
University of Tsukuba/Foundation for Advancement of International Science, Tsukuba, Ibaraki,
Japan
e-mail: ruike@fais.or.jp
K. Shibata
Yokohama National University, Yokohama, Kanagawa, Japan
e-mail: shibata-kenichi-ym@ynu.jp
K. Murakami (*)
Chiba Institute of Technology, Narashino, Chiba, Japan
e-mail: kazuhito.murakami@p.chibakoudai.jp
© Springer Nature Singapore Pte Ltd. 2020
Y. Inamori (ed.), Microcosm Manual for Environmental Impact Risk Assessment,
https://doi.org/10.1007/978-981-13-6798-4_6
45