concentration of DO is b, the oxygen transfer rate (δDO) at a certain DO concentration x can be calculated according to the following formula:
δDO ¼ a x À b
ð
Þ:
Since b ¼ 8.26 mg/L (Fig. 4.4) and a ¼ À0.00239 (Fig. 4.6), δDO (mg/L/min) in
this example is
δDO ¼ À0:00239 x À 8:26
ð
Þ :
This oxygen transfer rate (δDO) is used in Sect. 6.2, “Calculation of Respiration
and Production Quantities in the Microcosm System,” of this book.
4.3 Microcosm N-System
The maintenance culture of the microcosm N-system must remain aseptic. The
culture used for experimentation adhered to a culture maintenance method. The
inoculum dose of the N-system seed is 10 mL. A TP (Taub + peptone) medium
(shown in Table 3.1) is employed for cultivation. After adjusting the TP medium,
dispense it into a 300 mL Erlenmeyer flask by 200 mL volume, cap it with a silicon
plug, and then autoclave it at 121
C for 15 min to sterilize it. After allowing it to
cool to room temperature, add 10 mL of the N-system, which was cultured for
2–8 weeks, as a seed, and culture it in an incubator with an L/D cycle ¼ 12 hr/12 hr
and an illuminance ¼ 2400 lux (photosynthesis photon flux density ¼ 36 μmol/m
2 /
s), at a temperature of 25
C. Stir it lightly once a day until the culture begins
1–3 days later, then leave it at rest, and culture it afterward. Subculturing is
performed once every 2 months. Use a National (i.e., Panasonic) FL20SS-W/18 as
Fig. 4.6 Linear approximation with removal of measurement error
36
K. Shibata et al.
δDO ¼ a x À b
ð
Þ:
Since b ¼ 8.26 mg/L (Fig. 4.4) and a ¼ À0.00239 (Fig. 4.6), δDO (mg/L/min) in
this example is
δDO ¼ À0:00239 x À 8:26
ð
Þ :
This oxygen transfer rate (δDO) is used in Sect. 6.2, “Calculation of Respiration
and Production Quantities in the Microcosm System,” of this book.
4.3 Microcosm N-System
The maintenance culture of the microcosm N-system must remain aseptic. The
culture used for experimentation adhered to a culture maintenance method. The
inoculum dose of the N-system seed is 10 mL. A TP (Taub + peptone) medium
(shown in Table 3.1) is employed for cultivation. After adjusting the TP medium,
dispense it into a 300 mL Erlenmeyer flask by 200 mL volume, cap it with a silicon
plug, and then autoclave it at 121
C for 15 min to sterilize it. After allowing it to
cool to room temperature, add 10 mL of the N-system, which was cultured for
2–8 weeks, as a seed, and culture it in an incubator with an L/D cycle ¼ 12 hr/12 hr
and an illuminance ¼ 2400 lux (photosynthesis photon flux density ¼ 36 μmol/m
2 /
s), at a temperature of 25
C. Stir it lightly once a day until the culture begins
1–3 days later, then leave it at rest, and culture it afterward. Subculturing is
performed once every 2 months. Use a National (i.e., Panasonic) FL20SS-W/18 as
Fig. 4.6 Linear approximation with removal of measurement error
36
K. Shibata et al.