Chapter 4
Preparation of the Microcosm N-System
Ken-ichi Shibata, Kunihiko Kakazu, Kakeru Ruike,
and Kazuhito Murakami
Abstract In this chapter, the preparation of the microcosm test, including the
apparatus, the dissolved oxygen (DO) meters (electrode DO meter and fluorometric
DO meter), the calibration of the DO meter, and the oxygen dissolution rate are
described. Additionally, the treatment of the microcosm N-system from successive
subculturing is also explained.
4.1 Apparatus
• 300 mL Erlenmeyer flask
• Silicon plug (Matsuura Machinery, Catalog No. 6-343-16, Type No. C-40)
• Incubator (25
C, L/D cycle ¼ 12 hr/12 hr, illuminance ¼ 2400 lux (photosynthesis photon flux density ¼ 36 μmol/m
2 /s))
• Dissolved oxygen (DO) meters
• Black rubber tube (only needed when light leaks from DO meter)
• Stirrer (unnecessary if the DO meter is able to perform measurements in standing
water)
• Stirring rotor bar (unnecessary if the DO meter is able to perform measurements
in standing water)
K. Shibata (*)
Yokohama National University, Yokohama, Kanagawa, Japan
e-mail: shibata-kenichi-ym@ynu.jp
K. Kakazu
Foundation for Advancement of International Science, Tsukuba, Ibaraki, Japan
e-mail: kakazu@fais.or.jp
K. Ruike
University of Tsukuba/Foundation for Advancement of International Science, Tsukuba, Ibaraki,
Japan
e-mail: ruike@fais.or.jp
K. Murakami (*)
Chiba Institute of Technology, Narashino, Chiba, Japan
e-mail: kazuhito.murakami@p.chibakoudai.jp
© Springer Nature Singapore Pte Ltd. 2020
Y. Inamori (ed.), Microcosm Manual for Environmental Impact Risk Assessment,
https://doi.org/10.1007/978-981-13-6798-4_4
31
Preparation of the Microcosm N-System
Ken-ichi Shibata, Kunihiko Kakazu, Kakeru Ruike,
and Kazuhito Murakami
Abstract In this chapter, the preparation of the microcosm test, including the
apparatus, the dissolved oxygen (DO) meters (electrode DO meter and fluorometric
DO meter), the calibration of the DO meter, and the oxygen dissolution rate are
described. Additionally, the treatment of the microcosm N-system from successive
subculturing is also explained.
4.1 Apparatus
• 300 mL Erlenmeyer flask
• Silicon plug (Matsuura Machinery, Catalog No. 6-343-16, Type No. C-40)
• Incubator (25
C, L/D cycle ¼ 12 hr/12 hr, illuminance ¼ 2400 lux (photosynthesis photon flux density ¼ 36 μmol/m
2 /s))
• Dissolved oxygen (DO) meters
• Black rubber tube (only needed when light leaks from DO meter)
• Stirrer (unnecessary if the DO meter is able to perform measurements in standing
water)
• Stirring rotor bar (unnecessary if the DO meter is able to perform measurements
in standing water)
K. Shibata (*)
Yokohama National University, Yokohama, Kanagawa, Japan
e-mail: shibata-kenichi-ym@ynu.jp
K. Kakazu
Foundation for Advancement of International Science, Tsukuba, Ibaraki, Japan
e-mail: kakazu@fais.or.jp
K. Ruike
University of Tsukuba/Foundation for Advancement of International Science, Tsukuba, Ibaraki,
Japan
e-mail: ruike@fais.or.jp
K. Murakami (*)
Chiba Institute of Technology, Narashino, Chiba, Japan
e-mail: kazuhito.murakami@p.chibakoudai.jp
© Springer Nature Singapore Pte Ltd. 2020
Y. Inamori (ed.), Microcosm Manual for Environmental Impact Risk Assessment,
https://doi.org/10.1007/978-981-13-6798-4_4
31