C ≒ F, there is no influence; if in even one instance A6 ¼D, B6 ¼E, C6 ¼F, there is an
influence that may be attributed to the addition of the chemical substances.
Additionally, a condition shown in Fig. 7 is considered with respect to the
behavioral patterns when alien species are introduced into the microcosm. Of
these, a decreasing trend (Fig. 7a, b) is determined if there is no influence.
Fig. 5 Time course of
RCM after OTC addition to
microcosm N-system
Days
decomposer
producer
predator
A N/ml
B N/ml
C N/ml
control system
decomposer
producer
predator
(Number of individuals)
(Number of individuals)
Days
D N/ml
E N/ml
F N/ml
addition system
Evaluation
criteria
After addition, incubate for a certain period of time, and compare individual population of
each constituting microorganism with control system
A D
B E no effect
C F
A≠D
B≠E
C≠F
There is influence if either
one is applicable
The number of individuals should be counted at least two points at the time of addition (for example, 16 days
after the start of culture) and after a certain period of culture (for example, 16 days after addition), and the
change in useful microorganisms after addition is tracked as necessary.
addition
Fig. 6 Assessment from effect on indigenous microorganisms in microcosm
222
Appendices
influence that may be attributed to the addition of the chemical substances.
Additionally, a condition shown in Fig. 7 is considered with respect to the
behavioral patterns when alien species are introduced into the microcosm. Of
these, a decreasing trend (Fig. 7a, b) is determined if there is no influence.
Fig. 5 Time course of
RCM after OTC addition to
microcosm N-system
Days
decomposer
producer
predator
A N/ml
B N/ml
C N/ml
control system
decomposer
producer
predator
(Number of individuals)
(Number of individuals)
Days
D N/ml
E N/ml
F N/ml
addition system
Evaluation
criteria
After addition, incubate for a certain period of time, and compare individual population of
each constituting microorganism with control system
A D
B E no effect
C F
A≠D
B≠E
C≠F
There is influence if either
one is applicable
The number of individuals should be counted at least two points at the time of addition (for example, 16 days
after the start of culture) and after a certain period of culture (for example, 16 days after addition), and the
change in useful microorganisms after addition is tracked as necessary.
addition
Fig. 6 Assessment from effect on indigenous microorganisms in microcosm
222
Appendices