7.14.10 Pseudomonas putida MC (Decomposer)
Pseudomonas putida MC, one of the indigenous bacteria in the microcosm, was added
at 1, 10, and 100 times the abundance of indigenous bacteria in the microcosm, and its
influence on the structure and function of the ecosystem was examined. Pseudomonas
putida MC slowly decreased at 1-, 10-, or 100-times inoculation concentrations in
viable counts using the selective media method after microcosm inoculation, and the
abundance of microorganisms (in particular, its primary consumer, the protozoan
ciliate Cyclidium glaucoma) in the microcosm slowly increased. Pseudomonas putida
MC has been shown to be a suitable food source for microcosm heterotrophs. By
comparison, in N 30 , no significant difference was observed between the system with
Pseudomonas putida added and the system without it (control system).
7.14.11 Bacterial Mixed Culture
A mixed culture of four species of indigenous bacteria in the microcosm, Bacillus
cereus, Pseudomonas putida, Acinetobacter sp., and coryneform bacteria, was added
at 1, 10, and 100 times the abundance of indigenous bacteria in the microcosm, and
its influence on the structure and function of the ecosystem was examined. As a
result, the total number of bacteria CFU slowly decreased at 1-, 10-, or 100-times
inoculation concentrations in viable counts using the selective media method after
microcosm inoculation, and the abundance of microorganisms (in particular, its
primary consumer, the protozoan ciliate Cyclidium glaucoma) in the microcosm
slowly increased. The bacterial mixed culture has been shown to be a suitable food
source for microcosm heterotrophs. By comparison, in N 30 , no significant difference
was observed between the system with the bacterial mixed culture added and the
system without it (control system).
7.14.12 Pseudomonas putida prS2000 (Alien Species)
Pseudomonas putida prS2000 as alien species was added at 1, 10, and 100 times the
abundance of indigenous bacteria in the microcosm, and its influence on the structure and function of the ecosystem was examined. Pseudomonas putida prS2000
slowly decreased at 1-, 10-, or 100-times inoculation concentrations in viable counts
using the selective media method after microcosm inoculation, and the abundance of
microorganisms (in particular, its primary consumer, the protozoan ciliate Cyclidium
glaucoma) in the microcosm slowly increased. Pseudomonas putida prS2000 has
been shown to be a suitable food source for microcosm heterotrophs. By comparison, in N 30 , no significant difference was observed between the system with Pseudomonas putida added and the system without it (control system).
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153
Pseudomonas putida MC, one of the indigenous bacteria in the microcosm, was added
at 1, 10, and 100 times the abundance of indigenous bacteria in the microcosm, and its
influence on the structure and function of the ecosystem was examined. Pseudomonas
putida MC slowly decreased at 1-, 10-, or 100-times inoculation concentrations in
viable counts using the selective media method after microcosm inoculation, and the
abundance of microorganisms (in particular, its primary consumer, the protozoan
ciliate Cyclidium glaucoma) in the microcosm slowly increased. Pseudomonas putida
MC has been shown to be a suitable food source for microcosm heterotrophs. By
comparison, in N 30 , no significant difference was observed between the system with
Pseudomonas putida added and the system without it (control system).
7.14.11 Bacterial Mixed Culture
A mixed culture of four species of indigenous bacteria in the microcosm, Bacillus
cereus, Pseudomonas putida, Acinetobacter sp., and coryneform bacteria, was added
at 1, 10, and 100 times the abundance of indigenous bacteria in the microcosm, and
its influence on the structure and function of the ecosystem was examined. As a
result, the total number of bacteria CFU slowly decreased at 1-, 10-, or 100-times
inoculation concentrations in viable counts using the selective media method after
microcosm inoculation, and the abundance of microorganisms (in particular, its
primary consumer, the protozoan ciliate Cyclidium glaucoma) in the microcosm
slowly increased. The bacterial mixed culture has been shown to be a suitable food
source for microcosm heterotrophs. By comparison, in N 30 , no significant difference
was observed between the system with the bacterial mixed culture added and the
system without it (control system).
7.14.12 Pseudomonas putida prS2000 (Alien Species)
Pseudomonas putida prS2000 as alien species was added at 1, 10, and 100 times the
abundance of indigenous bacteria in the microcosm, and its influence on the structure and function of the ecosystem was examined. Pseudomonas putida prS2000
slowly decreased at 1-, 10-, or 100-times inoculation concentrations in viable counts
using the selective media method after microcosm inoculation, and the abundance of
microorganisms (in particular, its primary consumer, the protozoan ciliate Cyclidium
glaucoma) in the microcosm slowly increased. Pseudomonas putida prS2000 has
been shown to be a suitable food source for microcosm heterotrophs. By comparison, in N 30 , no significant difference was observed between the system with Pseudomonas putida added and the system without it (control system).
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153
