10-times quantity addition system, the light inside of the flask is not at full intensity,
and it is thought that the photosynthesis efficiency is low and counters the population
growth of phytoplankton. This is a photosynthesis inhibition phenomenon, similar to
algae in the natural ecosystem, and contributes to the extinction of fish from poor
oxygenation in lakes and marshes. It is thought that the decrease in the population of
Chlorella sp., which is a chlorophycean, is caused by a drop in their photosynthesis
efficiency. However, it becomes a P/R ratio ¼ 1 within each system, and it is thought
that the ecosystem function in a 10-times addition system is low, but the system is
maintained. From this, the 10-times quantity addition system was able to reproduce
the decrease in activity from algae in a natural aquatic ecosystem, but it is thought
that there is not recognized any influence such as the ecosystem collapses.
7.14.7 Scenedesmus quadricauda (Producer)
Scenedesmus quadricauda, one of the producers in the microcosm, was introduced
into the microcosm as the introduced species for bottom-up biomanipulation. As a
structural parameter, no species became extinct within a culture period. The population of Scenedesmus quadricauda decreased in the 1-times quantity addition
system from the time of addition to the 23rd day, and it increased afterward on the
30th day. In contrast, the population of Scenedesmus quadricauda in the 10-times
quantity addition system continued decreasing slightly from the time of addition to
the 30th day.
As a functional parameter, in the case of Scenedesmus quadricauda in a 1-times
quantity addition system, activity increased compared with the control system. This
is regarded as an effect through the improvement of the photosynthetic efficiency
from the increase in population. However, the DO just after the addition was in state
of high in activity in the 10-times addition system, but the amplitude of the wave
pattern decreased as time progressed, and an activity drop was shown. However, it
becomes a P/R ratio ¼ 1 within each system, and it is thought that the ecosystem
function that a 10-times quantity addition system has is low, but the system is
maintained. From this, the 10-times quantity addition system was able to reproduce
the decrease in activity from algae in a natural aquatic ecosystem, but it is thought
that there is no influence.
7.14.8 Tolypothrix sp. (Producer)
Tolypothrix sp., one of the producers in the microcosm, was introduced into the
microcosm as the introduced species for bottom-up biomanipulation. As a structural
parameter, each constituent organism class in the 1-times quantity addition system
remained in the system. Tolypothrix sp. maintained a population in the 10-times
quantity addition system until the 30th day, but Cyclidium glaucoma was not
observed after the 18th day. Aeolosoma hemprichi, Philodina erythrophthalma,
7 Example Assessments of the Microcosm N-System
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